Posters
Presentation Type
Poster
Discipline Track
Biomedical Science
Abstract Type
Research/Clinical
Abstract
Diabetic Retinopathy (DR) is the leading cause of blindness in the U.S. However, not much is known of its molecular pathway and how it attributes to increases in inflammatory response in the eye. One avenue we will investigate is the transforming growth factor beta (TGFB) signaling pathway and its effect of vascular endothelial growth factor (VEGF) secretion and cell viability. VEGF is the hallmark that exacerbates DR progression in prolonged diabetes. Some major concern that have arisen are the underlying effects of oxidants and antioxidants in elevating VEGF secretion in diabetes. In attempt to learn more, we evaluated how an oxidant (acrolein) and antioxidant (hypoxia) impact 661W cone photoreceptor cells in the retina. 661W cells were cultured in DMEM, 10% FBS, 1% AB and once confluent seeded into 6 wells with 300, 000 cells per well. Cells were conditioned in 5.5 and 30mM glucose, in addition to their appropriate treatments of hypoxia induce using cobalt chloride (CoCl2) and various concentrations of acrolein including 25, 50, 100, 200uM for a 24-hr. treatment period. Following the collection of conditioned media to measure VEGF secretion and cell viability to quantify number of viable cells using the hemocytometer. Moreover, to determine the role of TGFB signaling pathway inhibition will block the molecular pathway to determine how VEGF secretion and cell viability are affected in the respective treatments listed above.
Academic/Professional Position
Graduate Student
Mentor/PI Department
Molecular Science
Recommended Citation
Rodriguez, Reanna R., "Understanding an inflammatory pathway in Diabetic Retinopathy" (2023). Research Symposium. 104.
https://scholarworks.utrgv.edu/somrs/theme1/posters/104
Included in
Biology Commons, Cell and Developmental Biology Commons, Chemicals and Drugs Commons, Diseases Commons
Understanding an inflammatory pathway in Diabetic Retinopathy
Diabetic Retinopathy (DR) is the leading cause of blindness in the U.S. However, not much is known of its molecular pathway and how it attributes to increases in inflammatory response in the eye. One avenue we will investigate is the transforming growth factor beta (TGFB) signaling pathway and its effect of vascular endothelial growth factor (VEGF) secretion and cell viability. VEGF is the hallmark that exacerbates DR progression in prolonged diabetes. Some major concern that have arisen are the underlying effects of oxidants and antioxidants in elevating VEGF secretion in diabetes. In attempt to learn more, we evaluated how an oxidant (acrolein) and antioxidant (hypoxia) impact 661W cone photoreceptor cells in the retina. 661W cells were cultured in DMEM, 10% FBS, 1% AB and once confluent seeded into 6 wells with 300, 000 cells per well. Cells were conditioned in 5.5 and 30mM glucose, in addition to their appropriate treatments of hypoxia induce using cobalt chloride (CoCl2) and various concentrations of acrolein including 25, 50, 100, 200uM for a 24-hr. treatment period. Following the collection of conditioned media to measure VEGF secretion and cell viability to quantify number of viable cells using the hemocytometer. Moreover, to determine the role of TGFB signaling pathway inhibition will block the molecular pathway to determine how VEGF secretion and cell viability are affected in the respective treatments listed above.