Posters
Presentation Type
Poster
Discipline Track
Translational Science
Abstract Type
Research/Clinical
Abstract
Background: Rio Grande Valley (RGV) suffers from a high prevalence of certain cancers and lack the resources for accurate early diagnosis. Near-infrared (NIR) fluorescence-based imaging is a noteworthy and safer strategy for cancer detection compared to radiological imaging. There are several NIR dyes including indocyanine green (ICG) and its analogues that allow high-resolution and deep tissue imaging. However, these dyes possess some drawbacks, namely photo instability, toxicity, poor water solubility, and short half-lives. Chlorophyll (Chl) is a natural dietary and biocompatible NIR fluorescent substance which has the potential to serve as a cancer NIR imaging candidate. Hence, we aim to extract Chl from dietary leaves for cancer cell imaging.
Methods: 12 different dietary leaves were imaged using the IVIS imaging system at 600/710 nm to assess the fluorescence distribution of chlorophyll. Next, Chl dye was extracted using ethanol from the 6 most fluorescent leaves and visualized for fluorescence. Size distribution, surface charge, and the concentration of these extracts were measured by a DLS system. Chl internalization in AsPC-1 (pancreatic) and SK-HEP-1 (liver) cancer cell lines was determined by EVOS imaging system after treated with highest fluorescent extract at different concentrations.
Results: IVIS imaging data revealed that Chl was most fluorescent in bay leaf extract (4.98x1010 MFI). Physicochemical characterization of bay leaf extracted Chl indicated the particle size of 62.7 nm, zeta potential of -24.76 mV, and concentration at 1.11x1012 particles/mL. Cellular internalization data showed a dose dependent increase in bay leaf extracted Chl fluorescence in both cancer cell lines.
Conclusions: This data suggests that dietary Chl is a potent biocompatible alternative for cancer cells NIR fluorescent imaging.
Academic/Professional Position
Undergraduate
Academic/Professional Position (Other)
Junior
Mentor/PI Department
Immunology and Microbiology
Recommended Citation
Adriano, Benilde E.; Cotto, Nycol M.; Chauhan, Neeraj; Jaggi, Meena; Chauhan, Subhash C.; and Yallapu, Murali M., "A Natural Near-Infrared Fluorescent Probe for Cancer Cell Imaging" (2023). Research Symposium. 15.
https://scholarworks.utrgv.edu/somrs/theme1/posters/15
Included in
A Natural Near-Infrared Fluorescent Probe for Cancer Cell Imaging
Background: Rio Grande Valley (RGV) suffers from a high prevalence of certain cancers and lack the resources for accurate early diagnosis. Near-infrared (NIR) fluorescence-based imaging is a noteworthy and safer strategy for cancer detection compared to radiological imaging. There are several NIR dyes including indocyanine green (ICG) and its analogues that allow high-resolution and deep tissue imaging. However, these dyes possess some drawbacks, namely photo instability, toxicity, poor water solubility, and short half-lives. Chlorophyll (Chl) is a natural dietary and biocompatible NIR fluorescent substance which has the potential to serve as a cancer NIR imaging candidate. Hence, we aim to extract Chl from dietary leaves for cancer cell imaging.
Methods: 12 different dietary leaves were imaged using the IVIS imaging system at 600/710 nm to assess the fluorescence distribution of chlorophyll. Next, Chl dye was extracted using ethanol from the 6 most fluorescent leaves and visualized for fluorescence. Size distribution, surface charge, and the concentration of these extracts were measured by a DLS system. Chl internalization in AsPC-1 (pancreatic) and SK-HEP-1 (liver) cancer cell lines was determined by EVOS imaging system after treated with highest fluorescent extract at different concentrations.
Results: IVIS imaging data revealed that Chl was most fluorescent in bay leaf extract (4.98x1010 MFI). Physicochemical characterization of bay leaf extracted Chl indicated the particle size of 62.7 nm, zeta potential of -24.76 mV, and concentration at 1.11x1012 particles/mL. Cellular internalization data showed a dose dependent increase in bay leaf extracted Chl fluorescence in both cancer cell lines.
Conclusions: This data suggests that dietary Chl is a potent biocompatible alternative for cancer cells NIR fluorescent imaging.