Posters
Presentation Type
Poster
Discipline Track
Biomedical Science
Abstract Type
Research/Clinical
Abstract
Background: Diabetic cardiomyocytes alter their post-translational modification levels, especially in OGlcNAcylation and Phosphorylation. Insulin-like growth factor 1 (IGF-1) is a peptide known to induce favorable cardiovascular effects in patients with heart failure. Here, we focus on the downstream effects of IGF-1 as a potential DCM treatment.
Methods: H9c2 cells were cultured in DMEM-10% FBS at 80% of confluence. As a cellular model of cardiac hypertrophy, we used a high-glucose medium (30 mM glucose) in the presence or absence of 10 µmol/L of IGF-1 (HG and HG+IGF-1). As control groups, we used cells cultured in low-glucose DMEM (glucose 5mM) in the presence or absence of IGF-1 (LG and LG+IGF-1). After 48 hs of incubation, cell area was measured, and then proteins were extracted to analyze PTMs patterns by Western Blot. Cell area was measured to corroborate the effects of IGF-1 and high glucose concentration on hypertrophy induction.
Results: We found an increase in the area (a.u.) in the groups LG+IGF-1, HG, and HG + IGF-1 compared with the LG group (LG:1376±71; LG+IGF-1: 2247±116; HG:2215±105; HG+IGF-1: 2442±94). Also, the groups treated with IGF-1 showed a decrease in O-GlcNAcylation levels (a.u.) compared with the HG group (LG vs. HG vs. HG+IGF-1; vs. 189.4±20 vs. 132.1±12). Regarding the phospho-tyrosine modifications, IGF-1 significantly reduced the p-Tyr levels in the HG+IGF-1 group compared with control groups (LG vs LG+IGF-1 vs HG vs HG+IGF-1; LG:100.0±2.4; LG+IGF-1:89.45±6.5: HG:74.73±6.5; HG+IGF-1:44.45±5.6).
Results: These results show that IGF-1 signaling pathway stimulation lowers the levels of cardiac proteins O-GlcNAcylation and Tyrosine phosphorylation in high glucose
Academic/Professional Position
Staff
Academic/Professional Position (Other)
PhD
Recommended Citation
Medina, Andres; Trevino, Lizbeth; Salinas, Alejandra; Chaires, Yadira; and Rodriguez, Erick, "Effect of IGF-1 on post-translational modifications (PTMs) on a model of diabetes-induced cardiac hypertrophy in H9c2 cardiomyoblast cell line" (2023). Research Symposium. 46.
https://scholarworks.utrgv.edu/somrs/theme1/posters/46
Included in
Laboratory and Basic Science Research Commons, Medicine and Health Sciences Commons, Molecular Biology Commons
Effect of IGF-1 on post-translational modifications (PTMs) on a model of diabetes-induced cardiac hypertrophy in H9c2 cardiomyoblast cell line
Background: Diabetic cardiomyocytes alter their post-translational modification levels, especially in OGlcNAcylation and Phosphorylation. Insulin-like growth factor 1 (IGF-1) is a peptide known to induce favorable cardiovascular effects in patients with heart failure. Here, we focus on the downstream effects of IGF-1 as a potential DCM treatment.
Methods: H9c2 cells were cultured in DMEM-10% FBS at 80% of confluence. As a cellular model of cardiac hypertrophy, we used a high-glucose medium (30 mM glucose) in the presence or absence of 10 µmol/L of IGF-1 (HG and HG+IGF-1). As control groups, we used cells cultured in low-glucose DMEM (glucose 5mM) in the presence or absence of IGF-1 (LG and LG+IGF-1). After 48 hs of incubation, cell area was measured, and then proteins were extracted to analyze PTMs patterns by Western Blot. Cell area was measured to corroborate the effects of IGF-1 and high glucose concentration on hypertrophy induction.
Results: We found an increase in the area (a.u.) in the groups LG+IGF-1, HG, and HG + IGF-1 compared with the LG group (LG:1376±71; LG+IGF-1: 2247±116; HG:2215±105; HG+IGF-1: 2442±94). Also, the groups treated with IGF-1 showed a decrease in O-GlcNAcylation levels (a.u.) compared with the HG group (LG vs. HG vs. HG+IGF-1; vs. 189.4±20 vs. 132.1±12). Regarding the phospho-tyrosine modifications, IGF-1 significantly reduced the p-Tyr levels in the HG+IGF-1 group compared with control groups (LG vs LG+IGF-1 vs HG vs HG+IGF-1; LG:100.0±2.4; LG+IGF-1:89.45±6.5: HG:74.73±6.5; HG+IGF-1:44.45±5.6).
Results: These results show that IGF-1 signaling pathway stimulation lowers the levels of cardiac proteins O-GlcNAcylation and Tyrosine phosphorylation in high glucose