Posters
Presentation Type
Poster
Discipline Track
Clinical Science
Abstract Type
Research/Clinical
Abstract
Background: Colorectal cancer is the third most common cancer worldwide with the incidence rate of 1.8 million (10.2%) (GOBOCON-2018). CRC is endemic to Kashmir Valley due to both, kangri use and non-veg food habit. The current study was designed to explore the possible correlation between that FBXW7 and colorectal cancer progression.
Methods: FBXW7 gene mutations and expression was analyzed in 173 colorectal carcinoma tissues along with the adjacent non-cancerous matched tissues using polymerase chain reaction-single stranded confirmation polymorphism assay. Gene expression analysis was conducted using qRT-PCR, western blot and IHC.
Results: In total, six mutations were found in the FBXW7 gene, including four missense mutations, one frameshift deletion mutation, and one nonsense mutation. In expression analysis FBXW7 protein was found to be low in tumor tissues compared with matched normal tissues. Further, expression of FBXW7 was found highly significant with clinic-pathological characteristics like alcohol (p=0.006), dwelling (p= 0.004) and tumor stage (p=0.003). The overall expression of FBXW7 was low about 56.6% in tumor tissue samples.
Conclusion: A strong association was found between the low expression of FBXW7 and the progression of CRC. We highly recommend FBXW7 as a potential biomarker with therapeutic potential in CRC.
Academic/Professional Position
Other
Academic/Professional Position (Other)
PhD Student
Recommended Citation
Uroog, Laraib, "Mutational and expression analysis of FBXW7 gene in colorectal cancer patients among North Indian population" (2023). Research Symposium. 70.
https://scholarworks.utrgv.edu/somrs/theme1/posters/70
Included in
Mutational and expression analysis of FBXW7 gene in colorectal cancer patients among North Indian population
Background: Colorectal cancer is the third most common cancer worldwide with the incidence rate of 1.8 million (10.2%) (GOBOCON-2018). CRC is endemic to Kashmir Valley due to both, kangri use and non-veg food habit. The current study was designed to explore the possible correlation between that FBXW7 and colorectal cancer progression.
Methods: FBXW7 gene mutations and expression was analyzed in 173 colorectal carcinoma tissues along with the adjacent non-cancerous matched tissues using polymerase chain reaction-single stranded confirmation polymorphism assay. Gene expression analysis was conducted using qRT-PCR, western blot and IHC.
Results: In total, six mutations were found in the FBXW7 gene, including four missense mutations, one frameshift deletion mutation, and one nonsense mutation. In expression analysis FBXW7 protein was found to be low in tumor tissues compared with matched normal tissues. Further, expression of FBXW7 was found highly significant with clinic-pathological characteristics like alcohol (p=0.006), dwelling (p= 0.004) and tumor stage (p=0.003). The overall expression of FBXW7 was low about 56.6% in tumor tissue samples.
Conclusion: A strong association was found between the low expression of FBXW7 and the progression of CRC. We highly recommend FBXW7 as a potential biomarker with therapeutic potential in CRC.