Talks
Presentation Type
Oral Presentation
Discipline Track
Biomedical Science
Abstract Type
Research/Clinical
Abstract
Background: Cancer is one of the most common destructive diseases and the second leading cause of death in humans. Among cancer, cervical cancer is the second most common malignancy among women globally. Thus, there is a continuous need to search for chemotherapeutic chemicals or naturally occurring drugs to resolve this global health problem. Piperine (1-piperoylpeperdine) is present in the fruits of black pepper (Piper nigrum Linn.) and long pepper (Piper longum Linn.). It possesses several pharmacological properties and in the present study we have evaluated its anti-cancer potential on human cervical adenocarcinoma (HeLa) cells.
Methods: The anti-proliferative effect of piperine were investigated through some potent markers of apoptosis viz. reactive oxygen species (ROS) generation, cellular apoptosis and loss of mitochondrial membrane potential (MMP), DNA fragmentation, cell cycle kinetics, caspase-3 activity and cell migration against HeLa cells.
Results: The results showed that piperine exposure induces apoptosis significantly in a dose-dependent manner and inhibits the growth of HeLa cells with an increase in ROS generation, nuclear condensation and delayed wound healing. In addition, piperine also encourages cell death by the loss of MMP, DNA fragmentation and the activation of caspase-3. Growth inhibition of HeLa cells was found to be associated with G2/M phase arrest and sub-G1 accumulation.
Conclusions: The present study provides useful insight into the apoptotic potential of piperine and further in vivo and clinical studies will be needed for its validation and in the finding of more effective and least toxic regimens against cervical cancer.
Academic/Professional Position
Fellow
Academic/Professional Position (Other)
PhD Student
Recommended Citation
Jafri, Asif; Rais, Juhi; Kumar, Sudhir; and Arshad, Md, "Piperine encourages apoptosis in human cervical adenocarcinoma cells through ROS generation, DNA fragmentation, caspase-3 activation and cell cycle arrest" (2023). Research Symposium. 38.
https://scholarworks.utrgv.edu/somrs/theme1/track1/38
Included in
Alternative and Complementary Medicine Commons, Biotechnology Commons, Food Chemistry Commons, Medicinal and Pharmaceutical Chemistry Commons, Medicinal Chemistry and Pharmaceutics Commons, Molecular, Genetic, and Biochemical Nutrition Commons, Natural Products Chemistry and Pharmacognosy Commons, Other Pharmacology, Toxicology and Environmental Health Commons, Other Pharmacy and Pharmaceutical Sciences Commons, Pharmaceutics and Drug Design Commons, Pharmacology Commons
Piperine encourages apoptosis in human cervical adenocarcinoma cells through ROS generation, DNA fragmentation, caspase-3 activation and cell cycle arrest
Background: Cancer is one of the most common destructive diseases and the second leading cause of death in humans. Among cancer, cervical cancer is the second most common malignancy among women globally. Thus, there is a continuous need to search for chemotherapeutic chemicals or naturally occurring drugs to resolve this global health problem. Piperine (1-piperoylpeperdine) is present in the fruits of black pepper (Piper nigrum Linn.) and long pepper (Piper longum Linn.). It possesses several pharmacological properties and in the present study we have evaluated its anti-cancer potential on human cervical adenocarcinoma (HeLa) cells.
Methods: The anti-proliferative effect of piperine were investigated through some potent markers of apoptosis viz. reactive oxygen species (ROS) generation, cellular apoptosis and loss of mitochondrial membrane potential (MMP), DNA fragmentation, cell cycle kinetics, caspase-3 activity and cell migration against HeLa cells.
Results: The results showed that piperine exposure induces apoptosis significantly in a dose-dependent manner and inhibits the growth of HeLa cells with an increase in ROS generation, nuclear condensation and delayed wound healing. In addition, piperine also encourages cell death by the loss of MMP, DNA fragmentation and the activation of caspase-3. Growth inhibition of HeLa cells was found to be associated with G2/M phase arrest and sub-G1 accumulation.
Conclusions: The present study provides useful insight into the apoptotic potential of piperine and further in vivo and clinical studies will be needed for its validation and in the finding of more effective and least toxic regimens against cervical cancer.