Date of Award

Spring 5-7-2021

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Dr. Andrew Tsin

Second Advisor

Dr. Kristine Lowe

Third Advisor

Dr. Megan Keniry

Abstract

Diabetic retinopathy is an ocular disease which appears in patients who experience progression of diabetes mellitus over a continuous period of time. Oftentimes, patients remain undiagnosed through the first stages of diabetic retinopathy due to the fact that there is not a specific way to determine when a patient develops the disease. Ophthalmologists and other eye specialists diagnose a patient with diabetic retinopathy once the patient begins to show progressed symptoms of the disease. Previous experiments have been performed to increase our knowledge of diabetic retinopathy and early biomarkers of the disease. Several studies have determined the effects of diabetic retinopathy and apoptosis with cytochrome c presence using bovine retinal cells and rat models. The purpose of this experiment is to understand, analyze and quantify the effects of diabetic conditions on cytochrome c presence in the mitochondria of human retinal pericyte cells by using TUNEL, heme staining and Western Blot methods. In doing so, I will determine whether cytochrome c could serve as a potential biomarker in the early detection of diabetic retinopathy.

Included in

Biology Commons

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