α-Cyclodextrin decreases cholera toxin binding to GM1-gangliosides

Document Type

Article

Publication Date

7-2013

Abstract

Cholera toxin (CT), the principal virulence factor secreted by Vibrio cholerae, is an A-B5 type exotoxin that binds to host cell GM1-gangliosides and is responsible for cholera diarrhoea. We tested the hypothesis that the cyclic hexasaccharide α-cyclodextrin (α-CD), but not the cyclic heptasaccharides methyl-β-cyclodextrin (MD-β-CD) and hydroxypropyl-β-cyclodextrin (HP-β-CD) inhibit binding of CT to GM1-gangliosides. We report that α-CD decreases CT binding to GM1-ganglioside-coated microtitre plate wells and on the surface of fixed HeLa cells in a concentration-dependent manner, suggesting that this may be a promising lead for the development of compounds with therapeutic properties.

Comments

© 2013 SGM

Publication Title

Journal of medical microbiology

DOI

https://doi.org/10.1099/jmm.0.057232-0

Link to Full Text

Share

COinS