Theses and Dissertations - UTB/UTPA
Exploration of Nickel Tolerance and Hyperaccumulation in Arabidopsis thaliana Transformed with Streptanthus polygaloides Metal Tolerance Protein 1 (SpMTP1) cDNA
Date of Award
Master of Science (MS)
Dr. Michael Persans
Dr. Megan Keniry
Dr. Matthew Terry
This study evaluated the role of hyperaccumulator S. polygaloides Metal Tolerance Protein 1 (SpMTP1) in conferring Ni tolerance and accumulation in the non-accumulator Arabidopsis thaliana. Analysis for Ni content in transgenic Arabidopsis transformed with SpMTP1, following a 12-day treatment of 100 µM Ni, showed a significant increase in Ni concentration in comparison to the wild-type Arabidopsis and empty vector transformed plants. Exposure of transgenic Arabidopsis to nickel also resulted in a coordinated increase of antioxidant enzyme (superoxide dismutase, glutathione reductase, and catalase) activities in the transgenic Arabidopsis, suggesting that increased activities of these oxidative stress response (OSR) proteins may be due to SpMTP1 overexpression. These results demonstrate that expression of SpMTP1 in transgenic Arabidopsis can promote the transport and accumulation of nickel, as well as increase OSR enzymatic activities, which may serve as a plant model in improving plant candidates for use in the phytoextraction of nickel polluted soils.
University of Texas-Pan American
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