Theses and Dissertations - UTB/UTPA

Date of Award


Document Type


Degree Name

Master of Science (MS)



First Advisor

Dr. Michael Persans

Second Advisor

Dr. Megan Keniry

Third Advisor

Dr. Matthew Terry


This study evaluated the role of hyperaccumulator S. polygaloides Metal Tolerance Protein 1 (SpMTP1) in conferring Ni tolerance and accumulation in the non-accumulator Arabidopsis thaliana. Analysis for Ni content in transgenic Arabidopsis transformed with SpMTP1, following a 12-day treatment of 100 µM Ni, showed a significant increase in Ni concentration in comparison to the wild-type Arabidopsis and empty vector transformed plants. Exposure of transgenic Arabidopsis to nickel also resulted in a coordinated increase of antioxidant enzyme (superoxide dismutase, glutathione reductase, and catalase) activities in the transgenic Arabidopsis, suggesting that increased activities of these oxidative stress response (OSR) proteins may be due to SpMTP1 overexpression. These results demonstrate that expression of SpMTP1 in transgenic Arabidopsis can promote the transport and accumulation of nickel, as well as increase OSR enzymatic activities, which may serve as a plant model in improving plant candidates for use in the phytoextraction of nickel polluted soils.


Copyright 2015 Madeleine K. Sano. All Rights Reserved.

Granting Institution

University of Texas-Pan American

Included in

Biology Commons