Theses and Dissertations - UTB/UTPA
Use of randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) to distinguish North American populations of Acroptilon repens (L.) DC
Date of Award
Master of Science (MS)
Dr. Scott Gunn
Dr. Mohammed Farooqui
Dr. Robert I. Lonard
Sixty U.S. populations of Acroptilon repens collected in 1998 were analyzed using RAPD-PCR. Each population was represented by five specimens gathered from each location. A total of 301 specimens were examined using two Operon Technologies, RAPD-PCR primers (A 10 and C04). The DNA amplification products produced through the PCR process were electrophoretically separated to produce DNA fragments ranging from 100 to 2000 base-pairs in length. The 60 U.S. populations sampled, produce ten RAPD banding pattern types using A 10 primer and seventeen pattern types with C04. Genetic variation within and among the populations was low, however, one dendogram did show clustering of populations. Clonal reproduction is supported by the data.
Four of the ten A 10 primer patterns produced by 1998 U.S. Acroptilon repens populations were identical to patterns produced by samples from Kazakhstan and Uzbekistan. Approximately 75% of U.S. populations produce pattern A (A10) which is common to the above mentioned regions. This suggests a majority of the U.S. populations may have originated in Kazakhstan and Uzbekistan. The information provided by these results is currently being used to focus the foreign exploration for biological control agents for this weed.
University of Texas-Pan American
Copyright 1999 Raul A. Ruiz. All Rights Reserved.