Theses and Dissertations - UTB/UTPA

Date of Award


Document Type


Degree Name

Master of Science (MS)



First Advisor

Dr. Mohammed Farooqui

Second Advisor

Dr. Robert L. Mangan

Third Advisor

Dr. Robert I. Lonard


A spectrophotometric method for detection of phloxine B, a phototoxic dye proposed as a replacement for malathion bait sprays, in extracted tissues of Anastrepha ludens, Apis mellifera and in honey was developed. Dye detection was increased with a pH change from 6 to 13.7 in tissues or from 3.7 to 8 in honey with 2% sodium hydroxide. An LC 50 of 29.62 ppm phloxine B in 30% sucrose was obtained by feeding honey bees. A predictive model for dye in insect tissues and honey was developed and shown to be 89–92% effective. This study provides a forensic approach to determine if bees were killed or honey was contaminated by phloxine B sprays.


Copyright 2001 Aleena M. Tarshis Moreno. All Rights Reserved.

Granting Institution

University of Texas-Pan American