Theses and Dissertations - UTB/UTPA
Early Embryology of The Insect Oligotoma Saundersii (Embiidina) Using Targeted Genes as Developmental Landmarks
Date of Award
Master of Science (MS)
Dr. Matthew Terry
Dr. Kenneth R. Summy
Dr. Jonathan Lieman
Embiidina, or "webspinners", is a small, sexually dimorphic, polyneopterous insect order and is the only order to spin silk throughout their life cycle. Unique morphological features and the phylogenetic position of Embiidina make it an attractive subject for study of the evolution and development of the insect body plan and the origin of novel morphological features. Oligotoma saundersii is easily cultured and a viable laboratory organism. It has a fairly standard short-germ embryogenesis process requiring 453 hours at 28°C with progressive development beginning in the anterior region. Preliminary work on targeted developmental genes has yielded small portions of seven genes. ARACED-PCR is a novel, efficient method to amplify larger pieces of target genes using degenerate primers. This research represents the first detailed investigation of Embiidina embryology and one of few among the polyneopterous insects; therefore, it represents an important addition to our understanding of the evolution and development of insects.
University of Texas-Pan American
Copyright 2012 Omar Doria. All Rights Reserved.