Theses and Dissertations - UTB/UTPA

Date of Award


Document Type


Degree Name

Master of Science (MS)



First Advisor

Dr. Luis Materon

Second Advisor

Dr. Joanne Rampersad

Third Advisor

Dr. Mohammed Farooqui


Staphylococci from part of the natural flora of humans and some, like Staphylococcus aureus, appear to be evolving antibiotic resistance. The Lower Rio Grande Valley (LRGV) is an area of interest due to its proximity to the US/Mexico border where antibiotics could recently be purchased without prescription. Two libraries from community association (CA-S) and environmental association (E-S) have been collected and the staphylococci isolated. We hypothesize that their SCCmec type, which aids the staphylococci in antibiotic resistance, will not display classically defined association schemes and will surface in many species of staphylococci. This study generates antibiotic resistance profiles via antibiotic susceptibility testing, identifies the presence of Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus haemolyticus, the presence of the mecA gene, and SCCmec types I-V among both libraries through multiplex and standard polymerase chain reaction (PCR). The data produced is an insight into the composition of the staphylococci in the LRGV.


Copyright 2011 Diana Evelyn Treviño. All Rights Reserved.

Granting Institution

University of Texas-Pan American