Theses and Dissertations - UTB/UTPA
Isolation and characterization of polychlorinated biphenyl (PCB) degrading bacteria isolated from a PCB contaminated reservoir in Donna, TX
Date of Award
Master of Science (MS)
Dr. Luis Materon
Dr. Kristine Lowe
Dr. Michael Persans
The Donna Irrigation System (DIS) located in Donna, Hidalgo County, TX, was confirmed to be contaminated with polychlorinated biphenyls (PCBs) in 1993 and remediation of the site will be attempted in the future. The need to isolate novel PCB degrading bacteria for bioremediation has been an active area of research in recent decades; thus, it was hypothesized that a variety of microorganisms able to degrade PCBs could be isolated from sediments taken from the DIS. Two genes of the PCB degradation pathway, bphA1 and bphC, were detected in 43 bacterial isolates grown from DIS sediments using molecular and culture-based methodology, confirming the PCB degradation potential of these bacteria. Biochemical testing indicated differences in the enzymatic activity, carbohydrate fermentation ability, and carbon source utilization patterns of these isolates and strongly suggests that a variety of species, and possibly genera, of PCB degrading bacteria are present in the sediments of the DIS.
University of Texas-Pan American
Copyright 2014 Sergio Cepeda. All Rights Reserved.