
Mechanical Engineering Faculty Publications and Presentations
Document Type
Article
Publication Date
7-19-2024
Abstract
In this research, a novel electrochemical biosensor is proposed based on inducing graphene formation on polyimide substrate via laser engraving. Graphene polyaniline (G-PANI) conductive ink was synthesized by planetary mixing and applied to the working zone of the developed sensor to effectively enhance the electrical signals. The laser-induced graphene (LIG) sensor was used to detect alpha-fetoprotein (AFP) and 17β-Estradiol (E2) in the phosphate buffer saline (PBS) buffer and human serum. The electrochemical performance of the biosensor in determining these biomarkers was investigated by differential pulse voltammetry (DPV) and chronoamperometry (CA). In a buffer environment, alpha-fetoprotein (AFP) and 17β-Estradiol detection range were 4–400 ng/mL and 20–400 pg/mL respectively. The experimental results showed a limit of detection (LOD) of 1.15 ng/mL and 0.96 pg/mL for AFP and estrogen, respectively, with an excellent linear range (R2 = 0.98 and 0.99). In addition, the designed sensor was able to detect these two types of biomarkers in human serum successfully. The proposed sensor exhibited excellent reproducibility, repeatability, and good stability (relative standard deviation, RSD = 0.96%, 1.12%, 2.92%, respectively). The electrochemical biosensor proposed herein is easy to prepare and can be successfully used for low-cost, rapid detection of AFP and E2. This approach provides a promising platform for clinical detection and is advantageous to healthcare applications.
Recommended Citation
Tabassum, Ridma, Pritu Parna Sarkar, Ahmed Hasnain Jalal, Ali Ashraf, and Nazmul Islam. 2024. "Laser-Induced Electrochemical Biosensor Modified with Graphene-Based Ink for Label-Free Detection of Alpha-Fetoprotein and 17β-Estradiol" Polymers 16, no. 14: 2069. https://doi.org/10.3390/polym16142069
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Publication Title
Polymers
DOI
https://doi.org/10.3390/polym16142069
Comments
© 2024 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).