Hepatocellular carcinoma is the most common cancer of the liver and is the third leading cause of cancer deaths globally. Due to the limited serum biomarkers, detection of hepatocellular carcinoma usually occurs in the later, metastasized stages of the disease where the 5-year survival rate falls to around 3%. The identification of early diagnostic biomarkers for hepatocellular carcinoma is necessary to provide both improved prognostic outcomes and make treatment options dependent on liver dysfunction, such as resection and liver transplantation, available to patients. This study performed real-time polymerase chain reaction and western blot analysis for four hepatocellular carcinoma cell lines (SKHEP1, HEP3B, C3A, and HEPG2) to observe the expression of POTE-2 mRNA and protein. The data demonstrates significant overexpression of POTE-2 in HCC cell lines, indicating these HCC cell lines to be an ideal in-vitro model to study POTE-2 function in hepatocellular carcinoma.
Valdez, Alisha A.; Lopez, Samantha; Doxtater, Kyle; Ayala Pazzi, Ana; Sanchez, Amayrani; Leslie, Sophia; and Tripathi, Manish, "Characterization of POTE-2 Expression in Hepatocellular Carcinoma Cell Lines" (2022). MEDI 8127 Scholarly Activities Pre-Clerkship. 21.
Immunology and Microbiology