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Metastasis associated lung adenocarcinoma transcript 1 (MALAT 1) is a long non-coding RNA that has been implicated as a promotor of neovascularization in patients with diabetic retinopathy. The upregulation of vascular endothelial growth factor (VEGF) plays an important role in the pathogenesis of diabetic retinopathy by increasing angiogenesis, vasculogenesis, and vascular permeability. The purpose of this experiment is to investigate the relationship between MALAT 1 and VEGF in high and low glucose conditioned environment. Based on previous studies, MALAT 1 has been determined to be a possible target for treatment in patients with diabetic retinopathy. Further examination needs to be conducted to allow proper investigation and establishment of the effects of the relationship between MALAT 1 and VEGF in the pathophysiology of diabetic retinopathy. This study was carried out in Y-79 (Y79) cells. Cells were then transfected with Lipofectamine and Calcium Phosphate. After incubation for 18 hours, the cells were stimulated with complete RPMI. GFP expression was assessed 48hrs after transfection using Nikon confocal microscope.

Academic Level

medical student

Mentor/PI Department

Molecular Science

Available for download on Thursday, June 20, 2024