Extracellular Vesicles, and more specifically, exosomes, are essential for effective cell-to-cell communication in a wide variety of tissues. In the last couple of decades, these nanovesicles have been proven to be active participants and regulators in many disease processes; therefore, their therapeutic effects have been widely studied and proven in various cardiovascular diseases both, in vitro and in vivo. Thus, this study aims at assessing the effects of running healthy mice exosomes on cardiomyocyte and cardiac tissue samples obtained from diabetic mice. Here, we successfully extract exosomes from mice plasma and detect their presence through the use of anti-CD9 and anti-CD81 antibodies. Further work includes concentrating exosome presence and utilizing a wider variety of exosome-specific antibodies, as well as exploring techniques for more effective exosome extraction from plasma.
Garza, Miguel A.; Ramírez-Correa, Genaro A.; Garza-Rodríguez, Maria Lourdes; and Medina, Andres J., "Healthy Exosomes and their Effects on Diabetic Cardiomyocytes" (2022). MEDI 9331 Scholarly Activities Clinical Years. 62.
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