
School of Medicine Publications and Presentations
Document Type
Article
Publication Date
3-25-2015
Abstract
Although the past decade has seen tremendous progress in our understanding of fine-scale recombination, little is known about non-crossover (NCO) gene conversion. We report the first genome-wide study of NCO events in humans. Using SNP array data from 98 meioses, we identified 103 sites affected by NCO, of which 50/52 were confirmed in sequence data. Overlap with double strand break (DSB) hotspots indicates that most of the events are likely of meiotic origin. We estimate that a site is involved in a NCO at a rate of 5.9×10-6/bp/generation, consistent with sperm-typing studies, and infer that tract lengths span at least an order of magnitude. Observed NCO events show strong allelic bias at heterozygous AT/GC SNPs, with 68% (58–78%) transmitting GC alleles (P=5×10-4). Strikingly, in 4 of 15 regions with resequencing data, multiple disjoint NCO tracts cluster in close proximity (~20–30 kb), a phenomenon not previously seen in mammals.
Recommended Citation
Williams, A. L., Genovese, G., Dyer, T., Altemose, N., Truax, K., Jun, G., ... & Przeworski, M. (2015). Non-crossover gene conversions show strong GC bias and unexpected clustering in humans. Elife, 4, e04637. https://doi.org/10.7554/eLife.04637
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Publication Title
eLife
DOI
10.7554/eLife.04637
Academic Level
faculty
Mentor/PI Department
Office of Human Genetics
Comments
© 2015, Williams et al.
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