School of Medicine Publications and Presentations
Document Type
Article
Publication Date
3-4-2025
Abstract
Extracellular vesicles (EVs) are lipid-bound vesicles secreted by cells, including exosomes, microvesicles, and apoptotic bodies. Proteomic analyses of EVs, particularly in relation to cancer, reveal specific biomarkers crucial for diagnosis and therapy. However, isolation techniques such as ultracentrifugation, size-exclusion chromatography, and ultrafiltration face challenges regarding purity, contamination, and yield. Contamination from other proteins complicates downstream processing, leading to difficulties in identifying biomarkers and interpreting results. Future research will focus on refining EV characterization for diagnostic and therapeutic applications, improving proteomics tools for greater accuracy, and exploring the use of EVs in drug delivery and regenerative medicine. In this review, we provide a bird’s eye view of various challenges, starting with EV isolation methods, yield, purity, and limitations in the proteome analysis of EVs for identifying protein targets.
Recommended Citation
Singh, M., Tiwari, P. K., Kashyap, V., & Kumar, S. (2025). Proteomics of Extracellular Vesicles: Recent Updates, Challenges and Limitations. Proteomes, 13(1), 12. https://doi.org/10.3390/proteomes13010012
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Publication Title
Proteomes
DOI
10.3390/proteomes13010012
Academic Level
faculty
Mentor/PI Department
Immunology and Microbiology
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Medicine and Health Sciences Commons
Comments
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).