Tannic Acid Induces Endoplasmic Reticulum Stress-Mediated Apoptosis in Prostate Cancer

Authors

Prashanth K.B. Nagesh, The University of Texas Rio Grande Valley
Elham Hatami, University of Tennessee Health Science Center
Pallabita Chowdhury, University of Tennessee Health Science Center
Vivek Kumar Kashyap, The University of Texas Rio Grande Valley
Sheema Khan, The University of Texas Rio Grande Valley
Bilal B. Hafeez, The University of Texas Rio Grande ValleyFollow
Subhash C. Chauhan, The University of Texas Rio Grande ValleyFollow
Meena Jaggi, The University of Texas Rio Grande Valley
Murali M. Yallapu, The University of Texas Rio Grande ValleyFollow

Document Type

Article

Publication Date

3-2018

Abstract

Endoplasmic reticulum (ER) stress is an intriguing target with significant clinical importance in chemotherapy. Interference with ER functions can lead to the accumulation of unfolded proteins, as detected by transmembrane sensors that instigate the unfolded protein response (UPR). Therefore, controlling induced UPR via ER stress with natural compounds could be a novel therapeutic strategy for the management of prostate cancer. Tannic acid (a naturally occurring polyphenol) was used to examine the ER stress mediated UPR pathway in prostate cancer cells. Tannic acid treatment inhibited the growth, clonogenic, invasive, and migratory potential of prostate cancer cells. Tannic acid demonstrated activation of ER stress response (Protein kinase R-like endoplasmic reticulum kinase (PERK) and inositol requiring enzyme 1 (IRE1)) and altered its regulatory proteins (ATF4, Bip, and PDI) expression. Tannic acid treatment affirmed upregulation of apoptosis-associated markers (Bak, Bim, cleaved caspase 3, and cleaved PARP), while downregulation of pro-survival proteins (Bcl-2 and Bcl-xL). Tannic acid exhibited elevated G1 population, due to increase in p18INK4C and p21WAF1/CIP1 expression, while cyclin D1 expression was inhibited. Reduction of MMP2 and MMP9, and reinstated E-cadherin signifies the anti-metastatic potential of this compound. Altogether, these results demonstrate that tannic acid can promote apoptosis via the ER stress mediated UPR pathway, indicating a potential candidate for cancer treatment.

Comments

© 2018 by the authors

Recommended Citation

Nagesh, P., Hatami, E., Chowdhury, P., Kashyap, V. K., Khan, S., Hafeez, B. B., Chauhan, S. C., Jaggi, M., & Yallapu, M. M. (2018). Tannic Acid Induces Endoplasmic Reticulum Stress-Mediated Apoptosis in Prostate Cancer. Cancers, 10(3), 68. https://doi.org/10.3390/cancers10030068

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Publication Title

Cancers

DOI

10.3390/cancers10030068

Academic Level

faculty

Mentor/PI Department

Immunology and Microbiology