School of Podiatric Medicine - Medical Student Research

Document Type

Poster

Publication Date

Fall 8-3-2023

Abstract

Background: Recent studies have shown that fully reduced High Mobility Group Box-1 (FR-HMGB1) can improve wound healing under normoglycemic conditions, but it has not been tested for diabetic conditions. This study aimed to assess the regenerative potential of FR-HMGB1 under high glucose levels in vitro.

Methods: Migration of human fibroblasts (HF) was tested using a scratch assay in control conditions, and in the presence of FR-HMGB1 (1.0 µg/mL and 10 µg/mL), both in non-diabetic (ND) and diabetic (D) conditions (60mM of glucose added to the culture media). HF cells were tested after 18 hours of scratching and stained with actin green. The cell density in the scratch area was quantified using QuPath software. FR-HMGB1 treatment at both concentrations was also employed to assess the proliferative capacity of pre-osteoblasts in both ND and D conditions. MTS assay readings were performed at 48 hours of culture.

Results: Elevated glucose levels significantly inhibited the migration capacities of HF cells within the scratched area (ND-300.8±119 cells vs. D-136.3±19.55 cells). Both concentrations of HMGB1 enhanced HF migration under D conditions, while having no positive effect under ND cultures. Regarding pre-osteoblasts, 10µg of HMGB1 significantly increased percentile of cells in both ND (105±12.24 to 142±17.17) and D cultures (76.37±17.38 vs. 128.7±17.66).

Discussion/Conclusion: FR-HMGB1 enhanced HF migration under high glucose levels, while also rescuing the proliferation capacity of pre-osteoblasts. These findings suggest the potential of FR-HMGB1 as a therapeutic agent for improving tissue healing in diabetic wounds, offering new avenues for the treatment of impaired wound healing.

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