Posters
Presentation Type
Poster
Discipline Track
Translational Science
Abstract Type
Research/Clinical
Abstract
Background: Cervical cancer (CC) is one of the most common gynecological malignancies in the world, and human papillomavirus (HPV) infection is the most important risk factor for their development. Although there are methods for the early detection of CC and HPV infection, but there are not highly sensitive and specific, for it´s necessary to investigate alternatives such as miR-34a and MALAT1, implicated in the pathogenesis of CC. The objective was to evaluate the association of HPV status, viral load, the presence of coinfections, and the grade of CC precursor lesions with miR-34a and MALAT1 expression in patients with high and low-grade cervical lesions (CL) and patients without CL but HPV+.
Methods: Liquid-based cervical cytology (LBCC) specimens were obtained from 67 women diagnosed with low and high-grade CL, as well as LBCC HPV+, from which DNA and RNA were extracted. From DNA we genotyped and quantified the viral load for HPV 16, 18, and 51. From RNA, we performed a retrotranscription and evaluated the expression of MALAT1 (n=67) and miR-34a (n=29), all using a droplet-digital PCR assay. Statistical analysis was performed with SPSS 27.0 software using U Mann-Whitney and Kruskal-Wallis tests.
Results: We identified a statistically significant association between the under-expression of miR-34a, HPV+ status (p=0.010), coinfections (p=0.030), low (p =0.042), and high viral load (p=0.014), but not with the lesion grade. Also, MALAT1 overexpression was associated with HPV+ status (p=0.008) and high viral load (p =0.027), but not with co-infections or the grade of CC precursor lesions.
Conclusions: The expression of MALAT1 and miR-34a are associated with HPV status and viral load, but not with the grade of CC precursor lesions.
Academic/Professional Position
Graduate Student
Mentor/PI Department
Molecular Science
Recommended Citation
Solis-Coronado, Orlando; García-Quiñones, Juan A.; Oyervides-Muñoz, Mariel Aracely; Treviño, Victor; Sanchez-Dominguez, Celia N.; Pérez-Maya, Antonio A.; Perez-Ibave, Diana Cristina; Vidal-Gutierrez, Oscar; González-Guerero, Juan Francisco; Ramírez-Correa, Genaro A.; and Garza-Rodríguez, María Lourdes, "microRNA-34a and Long Non-Coding RNA MALAT1 Is Associated With HPV Status and Viral Load In Premalignant Cervical Lesions" (2023). Research Symposium. 82.
https://scholarworks.utrgv.edu/somrs/2022/posters/82
Included in
microRNA-34a and Long Non-Coding RNA MALAT1 Is Associated With HPV Status and Viral Load In Premalignant Cervical Lesions
Background: Cervical cancer (CC) is one of the most common gynecological malignancies in the world, and human papillomavirus (HPV) infection is the most important risk factor for their development. Although there are methods for the early detection of CC and HPV infection, but there are not highly sensitive and specific, for it´s necessary to investigate alternatives such as miR-34a and MALAT1, implicated in the pathogenesis of CC. The objective was to evaluate the association of HPV status, viral load, the presence of coinfections, and the grade of CC precursor lesions with miR-34a and MALAT1 expression in patients with high and low-grade cervical lesions (CL) and patients without CL but HPV+.
Methods: Liquid-based cervical cytology (LBCC) specimens were obtained from 67 women diagnosed with low and high-grade CL, as well as LBCC HPV+, from which DNA and RNA were extracted. From DNA we genotyped and quantified the viral load for HPV 16, 18, and 51. From RNA, we performed a retrotranscription and evaluated the expression of MALAT1 (n=67) and miR-34a (n=29), all using a droplet-digital PCR assay. Statistical analysis was performed with SPSS 27.0 software using U Mann-Whitney and Kruskal-Wallis tests.
Results: We identified a statistically significant association between the under-expression of miR-34a, HPV+ status (p=0.010), coinfections (p=0.030), low (p =0.042), and high viral load (p=0.014), but not with the lesion grade. Also, MALAT1 overexpression was associated with HPV+ status (p=0.008) and high viral load (p =0.027), but not with co-infections or the grade of CC precursor lesions.
Conclusions: The expression of MALAT1 and miR-34a are associated with HPV status and viral load, but not with the grade of CC precursor lesions.