Posters
Presentation Type
Poster
Discipline Track
Biomedical Science
Abstract Type
Research/Clinical
Abstract
Background: Biochanin A, an isoflavone that is mainly present in red clover, has potent chemopreventive properties against many cancers. Ovarian carcinoma is fifth most common and deadliest gynaecological malignancy that causes the highest mortality in females worldwide. Hence a substantial need for new therapies for combating this gynaecological malignancy arises.
Methods: The present study aimed to investigate anti-cancerous potentials of biochanin A on cultured human ovarian carcinoma PA-1 cells through the cell viability assay, cellular apoptosis, disruption of mitochondrial membrane potential (MMP), involvement of ROS, cell cycle kinetics, and expression of apoptosis-related genes namely, p53, Bax, Bcl-2, Noxa and Puma.
Results: The results of the experiments showed that Biochanin A significantly induces morphological alterations in PA-1 cells in a dose-dependent manner and thus inhibits cell to proliferate. Significant induction of apoptosis in PA-1 cells is evident by nuclear fragmentation, accumulation of ROS, loss of MMP, Annexin V positive cells and arrest of the cell cycle at G2/M phase. The up-regulated expression of apoptosis-related genes viz. p53, Bax, Noxa, Puma and down-regulation of Bcl-2 further signifies that biochanin A induces apoptosis in PA-1 cells
Conclusion: Biochanin A exhibited potent anti-cancerous properties against human ovarian carcinoma PA-1 cells by checking the proliferation and by inducing programmed cell death via accumulating ROS, changing membrane potential and by following p53 mediated apoptotic pathway.
Academic/Professional Position
Post-doc
Recommended Citation
Rais, Juhi; Jafri, Asif; Shivnath, Neelam; Khan, Habiba; and Arshad, Md, "International Conference on Cancer Health Disparities" (2023). Research Symposium. 62.
https://scholarworks.utrgv.edu/somrs/theme1/posters/62
Included in
Biology Commons, Cancer Biology Commons, Cell Biology Commons, Laboratory and Basic Science Research Commons, Natural Products Chemistry and Pharmacognosy Commons, Other Pharmacology, Toxicology and Environmental Health Commons, Toxicology Commons
International Conference on Cancer Health Disparities
Background: Biochanin A, an isoflavone that is mainly present in red clover, has potent chemopreventive properties against many cancers. Ovarian carcinoma is fifth most common and deadliest gynaecological malignancy that causes the highest mortality in females worldwide. Hence a substantial need for new therapies for combating this gynaecological malignancy arises.
Methods: The present study aimed to investigate anti-cancerous potentials of biochanin A on cultured human ovarian carcinoma PA-1 cells through the cell viability assay, cellular apoptosis, disruption of mitochondrial membrane potential (MMP), involvement of ROS, cell cycle kinetics, and expression of apoptosis-related genes namely, p53, Bax, Bcl-2, Noxa and Puma.
Results: The results of the experiments showed that Biochanin A significantly induces morphological alterations in PA-1 cells in a dose-dependent manner and thus inhibits cell to proliferate. Significant induction of apoptosis in PA-1 cells is evident by nuclear fragmentation, accumulation of ROS, loss of MMP, Annexin V positive cells and arrest of the cell cycle at G2/M phase. The up-regulated expression of apoptosis-related genes viz. p53, Bax, Noxa, Puma and down-regulation of Bcl-2 further signifies that biochanin A induces apoptosis in PA-1 cells
Conclusion: Biochanin A exhibited potent anti-cancerous properties against human ovarian carcinoma PA-1 cells by checking the proliferation and by inducing programmed cell death via accumulating ROS, changing membrane potential and by following p53 mediated apoptotic pathway.