Culturing embryonic cells from the parthenogenetic clonal marble crayfish Marmorkrebs Procambarus virginalis Lyko, 2017 (Decapoda: Astacidea: Cambaridae)

Authors

Heriberto DeLeon III, The University of Texas Rio Grande Valley
Juan Garcia Jr., The University of Texas Rio Grande Valley
Dionn Carlo Silva, The University of Texas Rio Grande Valley
Oscar Quintanilla, The University of Texas Rio Grande Valley
Zen Faulkes, The University of Texas Rio Grande ValleyFollow
John M. Thomas III, The University of Texas Rio Grande Valley

Document Type

Article

Publication Date

11-2019

Abstract

The parthenogenetic marbled crayfish, or Marmorkrebs (Procambarus virginalis Lyko 2017), is an emerging model organism. We describe a method to isolate cells from early-stage embryos and culture them in vitro. The identity of the cells was confirmed by sequencing the cytochrome c oxidase subunit I (COI) gene. This technique can be applied for use in the manipulation of embryonic parthenogenetic crayfish cells.

Comments

Original published version available at https://doi.org/10.1093/jcbiol/ruz063

Recommended Citation

Heriberto Deleon, III, Juan Garcia, Jr, Dionn Carlo Silva, Oscar Quintanilla, Zen Faulkes, John M Thomas, III, Culturing embryonic cells from the parthenogenetic clonal marble crayfish (Marmorkrebs) Procambarus virginalis Lyko, 2017 (Decapoda: Astacidea: Cambaridae), Journal of Crustacean Biology, Volume 39, Issue 6, November 2019, Pages 758–763, https://doi.org/10.1093/jcbiol/ruz063

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License

Publication Title

Journal of Crustacean Biology

DOI

10.1093/jcbiol/ruz063