The parthenogenetic marbled crayfish, or Marmorkrebs (Procambarus virginalis Lyko 2017), is an emerging model organism. We describe a method to isolate cells from early-stage embryos and culture them in vitro. The identity of the cells was confirmed by sequencing the cytochrome c oxidase subunit I (COI) gene. This technique can be applied for use in the manipulation of embryonic parthenogenetic crayfish cells.
Heriberto Deleon, III, Juan Garcia, Jr, Dionn Carlo Silva, Oscar Quintanilla, Zen Faulkes, John M Thomas, III, Culturing embryonic cells from the parthenogenetic clonal marble crayfish (Marmorkrebs) Procambarus virginalis Lyko, 2017 (Decapoda: Astacidea: Cambaridae), Journal of Crustacean Biology, Volume 39, Issue 6, November 2019, Pages 758–763, https://doi.org/10.1093/jcbiol/ruz063
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Journal of Crustacean Biology