Document Type
Article
Publication Date
3-7-2019
Abstract
Despite the central role PSD-95 plays in anchoring postsynaptic AMPARs, how PSD-95 itself is tethered to postsynaptic sites is not well understood. Here we show that the F-actin binding protein α-actinin binds to the very N-terminus of PSD-95. Knock-down (kd) of α-actinin phenocopies kd of PSD-95. Mutating lysine at position 10 or lysine at position 11 of PSD-95 to glutamate, or glutamate at position 53 or glutamate and aspartate at positions 213 and 217 of α-actinin, respectively, to lysine impairs in parallel PSD-95 binding to α-actinin and postsynaptic localization of PSD-95 and AMPARs. These experiments identify α-actinin as a critical PSD-95 anchor tethering the AMPAR - PSD-95 complex to postsynaptic sites.
Recommended Citation
Matt, Lucas, Karam Kim, Anne C. Hergarden, Tommaso Patriarchi, Zulfiqar A. Malik, Deborah K. Park, Dhrubajyoti Chowdhury et al. "α-Actinin anchors PSD-95 at postsynaptic sites." Neuron 97, no. 5 (2018): 1094-1109. https://doi.org/10.1016/j.neuron.2018.01.036
Publication Title
Neuron
DOI
10.1016/j.neuron.2018.01.036
Comments
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