α-Actinin Anchors PSD-95 at Postsynaptic Sites

Authors

Lucas Matt
Karam Kim
Anne E. Hergarden
Tommaso Patriarchi
Zulfiqar A. Malik
Deborah K. Park
Dhrubajyoti Chowdhury
Olivia R. Buonarati
Peter B. Henderson
Yonghong Zhang, The University of Texas Rio Grande ValleyFollow

Document Type

Article

Publication Date

3-7-2019

Abstract

Despite the central role PSD-95 plays in anchoring postsynaptic AMPARs, how PSD-95 itself is tethered to postsynaptic sites is not well understood. Here we show that the F-actin binding protein α-actinin binds to the very N-terminus of PSD-95. Knock-down (kd) of α-actinin phenocopies kd of PSD-95. Mutating lysine at position 10 or lysine at position 11 of PSD-95 to glutamate, or glutamate at position 53 or glutamate and aspartate at positions 213 and 217 of α-actinin, respectively, to lysine impairs in parallel PSD-95 binding to α-actinin and postsynaptic localization of PSD-95 and AMPARs. These experiments identify α-actinin as a critical PSD-95 anchor tethering the AMPAR - PSD-95 complex to postsynaptic sites.

Comments

PMC Copyright notice

Recommended Citation

Matt, Lucas, Karam Kim, Anne C. Hergarden, Tommaso Patriarchi, Zulfiqar A. Malik, Deborah K. Park, Dhrubajyoti Chowdhury et al. "α-Actinin anchors PSD-95 at postsynaptic sites." Neuron 97, no. 5 (2018): 1094-1109. https://doi.org/10.1016/j.neuron.2018.01.036

Publication Title

Neuron

DOI

10.1016/j.neuron.2018.01.036