Detection and Quantification of Nitrifying Bacteria Using Real-Time PCR

Authors

Engil Isadora Pujol pereira, The University of Texas Rio Grande ValleyFollow
Marcelo C. M. Teixeira Filho

Document Type

Book Chapter

Publication Date

10-9-2019

Abstract

Nitrification is the microbial-mediated transformation of ammonium (NH4 ⁺) into nitrate (NO3 ⁻). Many plant species depend on the availability of NO3 ⁻ as the main source of nitrogen (N). On the other hand, because NO3 ⁻ is highly mobile in the soil profile, its excess concentration can cause environmental pollution. Nitrification can be estimated at the process level, but with the development of molecular techniques it is also possible to estimate the abundance of nitrifying bacteria in the soil. Hence, in this chapter we describe the procedure for detection and quantification of nitrifying bacteria in soil samples using real-time quantitative polymerase chain reaction (PCR).

Comments

© 2020 Springer Science+Business Media, LLC, part of Springer Nature

Recommended Citation

Pereira, E.I.P., Teixeira Filho, M.C.M. (2020). Detection and Quantification of Nitrifying Bacteria Using Real-Time PCR. In: Gupta, K. (eds) Nitrogen Metabolism in Plants. Methods in Molecular Biology, vol 2057. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9790-9_13

Publication Title

Nitrogen Metabolism in Plants

DOI

https://doi.org/10.1007/978-1-4939-9790-9_13