Theses and Dissertations
Nannochloris sp. and Oocystis sp. Algae Exposed to Osmotic Stress for Increased Lipid Production for Use in Biofuels
Date of Award
Master of Science (MS)
Dr. Michael Persans
Dr. Hudson DeYoe
Dr. Megan Keniry
Algal biofuel research is important due to the potential need for replacement of non-renewable fossil fuels and a changing global climate. Under stress, algal cells tend to increase neutral lipid production, which are a suitable source for biofuels. The goal of this study was to subject Nannochloris sp.. and Oocystis sp. algal cells to osmotic stress by modifying sucrose and salinity levels in different treatment medias.
The treatment containing the lowest salinity (15 ppt) and no sucrose yielded the highest lipid fluorescence/cell in Nannochloris sp.. cells (0.00142 ± 0.00044) on day 2 while the treatment containing highest salinity (35ppt) and no sucrose yielded the highest lipid fluorescence/cell in Oocystis sp. cells (0.0042 ± 0.00068) on day 2. The highest lipid fluorescence/cell occurs within the first seven days for both Nannochloris sp.. and Oocystis sp. and the lipid fluorescence/cell reaches a plateau after 14 days in culture for all treatments tested.
Chavez, Laura M., "Nannochloris sp. and Oocystis sp. Algae Exposed to Osmotic Stress for Increased Lipid Production for Use in Biofuels" (2018). Theses and Dissertations. 254.
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