Theses and Dissertations
Identification of Inhibitors of the Glutaminyl and Prolyl tRNA Synthetases from Pseudomonas aeruginosa
Date of Award
Master of Science (MS)
Dr. James Bullard
Dr. Robert Dearth
Dr. Megan Keniry
Antibiotic resistance is a growing global threat and bacterial infections caused by antibiotic resistant bacteria have become increasingly difficult and expensive to treat with most current antibiotics. Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen and a leading cause of nosocomial infections and is the leading cause of mortality in cystic fibrosis patients. Aminoacyl-tRNA synthetases (aaRSs) are a class of enzymes that catalyze the covalent attachment of amino acids to their cognate tRNAs during protein biosynthesis. The glutaminyl- tRNA synthetase from P. aeruginosa was over-expressed, enzymatically characterized and developed into a screening platform for the discovery of chemical compounds that inhibit protein synthesis. Using scintillation proximity assay (SPA) technology GlnRS and ProRS from P. aeruginosa were each used as a screening platform for the detection of compounds that inhibited the enzymatic activity of either PaGlnRS or PaProRS.
Escamilla, Yaritza, "Identification of Inhibitors of the Glutaminyl and Prolyl tRNA Synthetases from Pseudomonas aeruginosa" (2019). Theses and Dissertations. 451.
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