Theses and Dissertations

Date of Award


Document Type


Degree Name

Master of Science (MS)


Ocean, Coastal, and Earth Sciences

First Advisor

Dr. MD Saydur Rahman

Second Advisor

Dr. Jude Benavides

Third Advisor

Dr. Abdullah Faiz Rahman


Global warming due to climate change is predicted to intensify the heat stress in marine and coastal organisms, affecting their development, growth and reproductive functions. In this study. I analyzed gonadal development, heat shock protein 70 (HSP70), nitrotyrosine protein (NTP), dinitrophenyl (DNP) expressions, cellular apoptosis, and coelomic fluid (CF) conditions in American oyster. Oysters were placed in six aquariums and exposed to control (24°C), medium (28°C), and high (32°C) temperatures for one week. Higher temperature significantly decreased the number of eggs and sperm. CF protein concentrations also declined compared to control. In contrast, CF pH and HSP70 expression in gonad increased after heat-exposure, consistent with increased gonadal apoptosis. The enhanced apoptosis in gonads of heat-exposed oysters was associated with increased NTP and DNP expressions in gametes. Collectively, these results suggest that higher temperatures drastically increased oxidative stress leading to increased cellular apoptosis, which subsequently declined gonadal functions in American oyster.


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