Jatropha curcas protoplast isolation and inhibitory effects of culture

Author

Christopher S. Lukaszewski, The University of Texas Rio Grande Valley

Date of Award

5-2016

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Dr. Frederic Zaidan

Second Advisor

Dr. Robert Edwards

Third Advisor

Dr. Matthew Terry

Abstract

Improvement on the sub-tropical plant Jatropha curcas may increase the use of this drought tolerant oilseed crop for the production of biodiesel. One of the methods for enhancing a species is through genetic manipulation of the protoplast. Isolating protoplasts is challenging and culturing has many obstacles similar to those seen in tissue culture, such as oxidative browning. Successful isolation of J. curcas leaf protoplasts resulted in an average of 3.23 x 106 viable protoplasts/mL. However, protoplasts diminished within 2 days after culturing. A review of the potential solutions used for overcoming the inhibitory effects of culture may reveal further insight on the culturing of J. curcas protoplasts.

Comments

Copyright 2016 Christopher S. Lukaszewski. All Rights Reserved.

https://www.proquest.com/dissertations-theses/i-jatropha-curcas-protoplast-isolation-inhibitory/docview/1810996032/se-2?accountid=7119

Recommended Citation

Lukaszewski, Christopher S., "Jatropha curcas protoplast isolation and inhibitory effects of culture" (2016). Theses and Dissertations. 59.
https://scholarworks.utrgv.edu/etd/59