Theses and Dissertations

Date of Award


Document Type


Degree Name

Master of Science (MS)



First Advisor

Dr. Nirakar Sahoo

Second Advisor

Dr. Rupesh Kariyat

Third Advisor

Dr. Bradley Christoffersen


Chapter 2: In this chapter, I have provided an update on recent developments and advances on early signaling events in plant-herbivore interactions, with a particular emphasis on the membrane potential changes (Vm), calcium (Ca2+), reactive oxygen species (ROS) and plant ion channels involvement in early signaling events initiation and propagation of defense signaling cascade.

Chapter 3: In this chapter, we show that tobacco hornworm (Manduca sexta) caterpillar oral secretion (OS) induces ROS in tomato (Solanum lycopersicum) protoplasts. By using a dye-based ROS imaging approach, our study showed that the application of Plant-Fed (PF) M. sexta OS generates significantly higher ROS while artificial Diet-Fed (DF) M. sexta OS failed to induce ROS in isolated tomato protoplasts.

Chapter 4: In this chapter, we demonstrate that hemolymph from M. sexta also induce ROS and Ca2+ and thereby act as an herbivore associated elicitor (HAE). Using a dye-based imaging technique, our study showed that the application of crude M. sexta hemolymph potently increased ROS as well as Ca2+ production in isolated tomato protoplasts.

In summary, our results demonstrate that M. sexta OS and hemolymph induces cellular defense signals by modulating intracellular ROS and Ca2+ in tomato protoplast.


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