Theses and Dissertations

Date of Award


Document Type


Degree Name

Master of Science (MS)


Ocean, Coastal, and Earth Sciences

First Advisor

Dr. MD Saydur Rahman

Second Advisor

Dr. Amit Raysoni

Third Advisor

Dr. Lucia Carreon Martinez


Aquatic ecosystems are inundated by environmental pesticides. This study, observed the dose-dependent effects of pesticides cocktail (metalachlor, linuron, isoproturon, tebucanazole, aclonifen, atrazine, pendimethalin, and azinphos-methyl) and elevated temperature (32 °C for 4-week exposure) on morphology of gills and kidneys, and expression of nitrotyrosine protein (NTP), dinitrophenyl protein (DNP), catalase (CAT), superoxidase dismutase (SOD), Na+/K+-ATPase, renin, and apoptosis in tissues of goldfish. Additionally, the effects on the free-swimming behavior were also observed. Histological analysis showed wide-spread damage in tissues at higher temperature and pesticides co-exposure. Immunohistochemical analysis demonstrated alterations in NTP, DNP, CAT and SOD expressions in tissues at higher temperature and pesticides co-exposure. Expression of renin and Na+/K+-ATPase was decreased with increasing dose of pesticides. Collectively, our results suggest that the combined effects of heat stress and pesticides alter swimming behavior, cause cellular damages, and alter osmoregulatory and antioxidant enzyme expressions in goldfish.


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