Health & Biomedical Sciences Faculty Publications and Presentations
Document Type
Article
Publication Date
1-2011
Abstract
Functional properties of large conductance Ca2+ activated potassium (BK) channels are determined by complex alternative splicing of the Kcnma1 gene encoding the alpha pore-forming subunit. Inclusion of the STREX exon in a C-terminal splice site is dynamically regulated and confers enhanced Ca2+ sensitivity and channel inhibition via cAMP-dependent phosphorylation. Here, we describe a real time quantitative PCR (qPCR) approach to investigate relative changes in the expression of STREX and ZERO splice variants using a newly designed set of probes and primers for TaqMan-based qPCR analysis of cDNA from the rat dentate gyrus at different time points following pilocarpine-induced status epilepticus. Reduction in Kcnma1 gene expression is associated with a relative increase of STREX splice variant. Relative expression of STREX variant mRNA was increased at 10 days and at more than 1 month following status epilepticus. The biological consequences of seizure-related changes in alternative splicing of Kcnma1 deserve additional investigation.
Recommended Citation
Ermolinsky, Boris S., Frank Skinner, Ileana Garcia, Massoud F. Arshadmansab, Luis F. Pacheco Otalora, Masoud M. Zarei, and Emilio R. Garrido-Sanabria. "Upregulation of STREX splice variant of the large conductance Ca2+-activated potassium (BK) channel in a rat model of mesial temporal lobe epilepsy." Neuroscience research 69, no. 1 (2011): 73-80. https://doi.org/10.1016/j.neures.2010.09.011
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Publication Title
Neuroscience Research
DOI
10.1016/j.neures.2010.09.011
Comments
© 2010 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved. Original published version available at https://doi.org/10.1016/j.neures.2010.09.011