Characterization of SNP markers for the painted bunting (Passerina ciris) and their relevance in population differentiation and genome evolution studies
Document Type
Article
Publication Date
3-15-2019
Abstract
We developed nuclear single nucleotide polymorphism (SNP) markers for the Painted Bunting (Passerina ciris) using next-generation sequencing genotyping on the Illumina HiSeq4000 platform. Following the best-RAD approach, we sequenced 96 individuals from five breeding populations occurring in the United States. We assessed the discerning power of 105,000 SNPs and confirmed the quality of these markers in population differentiation studies. We detected outlier SNPs within or near protein coding genes, including sequences coding for olfactory receptors which may relate to migratory navigation. Finally, we provide a novel list of 33 primer pairs flanking SNPs to link individuals to regional populations and improve our understanding of the evolution of this migratory songbird.
Recommended Citation
Contina, Andrea, Rachael A. Bay, Vinh Le Underwood, Thomas B. Smith, Jeffrey F. Kelly, Eli S. Bridge, and Kristen C. Ruegg. "Characterization of SNP markers for the Painted Bunting (Passerina ciris) and their relevance in population differentiation and genome evolution studies." Conservation Genetics Resources 11 (2019): 5-10. https://doi.org/10.1007/s12686-017-0950-7
Publication Title
Conservation Genetics Resources
DOI
https://doi.org/10.1007/s12686-017-0950-7
Comments
Reprints and permissions
https://rdcu.be/dPMWB