Theses and Dissertations - UTB/UTPA
Date of Award
2000
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Biology
First Advisor
Dr. Mohammed Farooqui
Second Advisor
Dr. Robert I. Lonard
Third Advisor
Dr. K. Daniel Murray
Abstract
The activities of mouse liver glutathione S-transferases (GSTs) induced by myristicin, the active compound in parsley leaf oil, against the carcinogens 4-nitroquinoline 1-oxide (4NQO) and benzo(a)pyrene-4,5-oxide (BP-4,5-oxide) were studied. A/J mice were treated with three 20 mg doses (one dose every other day) by gavage. Affinity purified liver GST samples from treated animals showed a 7.2-fold increase in activity toward 4NQO when compared with equal amounts of liver samples prepared from the control group. Activity against BP-4,5-oxide was increased 1.8-fold in the experimental group. Experimental samples also showed a significant increase in activity toward the substrates 1-chloro-2,4-dinitrobenzene (CDNB), 2,4-dichloronitrobenzene (DCNB), ethacrynic acid (E-Acid), and cumene hydroperoxide. These results suggested an increase in the three major classes of GSTs α, μ, and π and was further substantiated through Western blot analysis. Based on our results, myristicin could be considered an important chemopreventive agent against cancer.
Granting Institution
University of Texas-Pan American
Comments
Copyright 2000 Eduardo Saldivar. All Rights Reserved.
https://www.proquest.com/dissertations-theses/detoxification-4-nitroquinoline-1-oxide-benzo/docview/230629879/se-2?accountid=7119