Theses and Dissertations - UTB/UTPA

Date of Award


Document Type


Degree Name

Master of Science (MS)



First Advisor

Dr. Mohammed Farooqui

Second Advisor

Dr. Robert I. Lonard

Third Advisor

Dr. K. Daniel Murray


The activities of mouse liver glutathione S-transferases (GSTs) induced by myristicin, the active compound in parsley leaf oil, against the carcinogens 4-nitroquinoline 1-oxide (4NQO) and benzo(a)pyrene-4,5-oxide (BP-4,5-oxide) were studied. A/J mice were treated with three 20 mg doses (one dose every other day) by gavage. Affinity purified liver GST samples from treated animals showed a 7.2-fold increase in activity toward 4NQO when compared with equal amounts of liver samples prepared from the control group. Activity against BP-4,5-oxide was increased 1.8-fold in the experimental group. Experimental samples also showed a significant increase in activity toward the substrates 1-chloro-2,4-dinitrobenzene (CDNB), 2,4-dichloronitrobenzene (DCNB), ethacrynic acid (E-Acid), and cumene hydroperoxide. These results suggested an increase in the three major classes of GSTs α, μ, and π and was further substantiated through Western blot analysis. Based on our results, myristicin could be considered an important chemopreventive agent against cancer.


Copyright 2000 Eduardo Saldivar. All Rights Reserved.

Granting Institution

University of Texas-Pan American

Included in

Biology Commons