Theses and Dissertations - UTB/UTPA
Date of Award
5-2012
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Chemistry
First Advisor
Dr. Elamin E. Ibrahim
Second Advisor
Dr. James Bullard
Third Advisor
Dr. Hassan Ahmad
Abstract
Bacillus thuringiensis (Bt) produces the non specific Cyt protein and the specific Cry protein. Past research has shown these proteins are toxic to insects, red blood cells, cancer cells, and some bacteria. While many antibacterial substances are known to exist, efforts to find new ones still continue. In this project we used Bt 6-2-56 (containing a novel filament associated parasporal body which appears non-toxic to insects and protozoan) and Bt 4Q13 (which has been shown to have activity against E. coli and S. aureus) to develop a simple, easy, scalable assay in a 96 well Microtitre plate to look for Bt strains with antibacterial activity. The results showed that 6-2-56 may be toxic to E. coli and may be used as positive control to screen Bt libraries for antibacterial activity. Solubilization in phosphate buffer (pH 7.5) or water, activation with proteases of Bt 2-56 proteins abolished the antibacterial effect.
Granting Institution
University of Texas-Pan American
Comments
Copyright 2012 Auriole T. Tamegnon. All Rights Reserved.
https://www.proquest.com/dissertations-theses/development-assay-screen-novel-proteins-i/docview/1024431527/se-2