Theses and Dissertations - UTB/UTPA
Development of An Assay to Screen for Novel Proteins from Bacillus Thuringiensis with Antibacterial Activity
Date of Award
Master of Science (MS)
Dr. Elamin E. Ibrahim
Dr. James Bullard
Dr. Hassan Ahmad
Bacillus thuringiensis (Bt) produces the non specific Cyt protein and the specific Cry protein. Past research has shown these proteins are toxic to insects, red blood cells, cancer cells, and some bacteria. While many antibacterial substances are known to exist, efforts to find new ones still continue. In this project we used Bt 6-2-56 (containing a novel filament associated parasporal body which appears non-toxic to insects and protozoan) and Bt 4Q13 (which has been shown to have activity against E. coli and S. aureus) to develop a simple, easy, scalable assay in a 96 well Microtitre plate to look for Bt strains with antibacterial activity. The results showed that 6-2-56 may be toxic to E. coli and may be used as positive control to screen Bt libraries for antibacterial activity. Solubilization in phosphate buffer (pH 7.5) or water, activation with proteases of Bt 2-56 proteins abolished the antibacterial effect.
University of Texas-Pan American
Copyright 2012 Auriole T. Tamegnon. All Rights Reserved.