Theses and Dissertations - UTB/UTPA

Date of Award


Document Type


Degree Name

Master of Science (MS)



First Advisor

Dr. Elamin E. Ibrahim

Second Advisor

Dr. James Bullard

Third Advisor

Dr. Hassan Ahmad


Bacillus thuringiensis (Bt) produces the non specific Cyt protein and the specific Cry protein. Past research has shown these proteins are toxic to insects, red blood cells, cancer cells, and some bacteria. While many antibacterial substances are known to exist, efforts to find new ones still continue. In this project we used Bt 6-2-56 (containing a novel filament associated parasporal body which appears non-toxic to insects and protozoan) and Bt 4Q13 (which has been shown to have activity against E. coli and S. aureus) to develop a simple, easy, scalable assay in a 96 well Microtitre plate to look for Bt strains with antibacterial activity. The results showed that 6-2-56 may be toxic to E. coli and may be used as positive control to screen Bt libraries for antibacterial activity. Solubilization in phosphate buffer (pH 7.5) or water, activation with proteases of Bt 2-56 proteins abolished the antibacterial effect.


Copyright 2012 Auriole T. Tamegnon. All Rights Reserved.

Granting Institution

University of Texas-Pan American