Physics & Astronomy Faculty Publications
Document Type
Article
Publication Date
4-3-2015
Abstract
We report the first in vitro enzymatic synthesis of paramagnetic and antiferromagnetic nanoparticles toward magnetic ELISA reporting. With our procedure, alkaline phosphatase catalyzes the dephosphorylation of L-ascorbic-2-phosphate, which then serves as a reducing agent for salts of iron, gadolinium, and holmium, forming magnetic precipitates of Fe45±14Gd5±2O50±15 and Fe42±4Ho6±4O52±5. The nanoparticles were found to be paramagnetic at 300 K and antiferromagnetic under 25 K. Although weakly magnetic at 300 K, the room-temperature magnetization of the nanoparticles found here is considerably greater than that of analogous chemically-synthesized LnxFeyOz (Ln = Gd, Ho) samples reported previously. At 5 K, the nanoparticles showed a significantly higher saturation magnetization of 45 and 30 emu/g for Fe45±14Gd5±2O50±15 and Fe42±4Ho6±4O52±5, respectively. Our approach of enzymatically synthesizing magnetic labels reduces the cost and avoids diffusional mass-transfer limitations associated with pre-synthesized magnetic reporter particles, while retaining the advantages of magnetic sensing.
Recommended Citation
Kolhatkar, Arati G., Chamath Dannongoda, Katerina Kourentzi, Andrew C. Jamison, Ivan Nekrashevich, Archana Kar, Eliedonna Cacao et al. "Enzymatic synthesis of magnetic nanoparticles." International journal of molecular sciences 16, no. 4 (2015): 7535-7550. http://doi.org/10.3390/ijms16047535
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
First Page
7535
Last Page
7550
Publication Title
International Journal of Molecular Sciences
DOI
10.3390/ijms16047535
Comments
© 2015 by the authors; licensee MDPI, Basel, Switzerland. Original version available at: http://doi.org/10.3390/ijms16047535