Physics & Astronomy Faculty Publications
Document Type
Article
Publication Date
3-24-2026
Abstract
The broadly conserved ParB protein performs crucial functions in bacterial chromosome segregation and replication regulation. The cellular function of ParB requires it to dimerize, recognize parS DNA sequences, clamp on DNA, then slide to adjacent sequences through nonspecific DNA binding. How ParB coordinates nonspecific DNA binding and sliding remains elusive. Here, we combine multiple in vitro biophysical and computational tools and in vivo approaches to address this question. We found that five conserved lysine residues in the C-terminal domain (CTD) of ParB play distinct roles. While the two central rigid lysine residues provide the structural platform for the CTD, the three peripheral flexible lysine residues are implicated in efficient ParB sliding. Mutations in each individual lysine decreased ParB’s DNA compaction capabilities, indicating that all five lysine residues are critical for properly positioning the DNA along the ParB CTD surface. Importantly, the integrity of these five lysines is crucial for ParB’s in vivo functions, including fluorescence foci formation and sporulation initiation. Many proteins with diverse cellular activities need to move along DNA while loosely bound. Our findings provide molecular insight into how the fast backbone dynamics of multiple basic residues enable DNA-binding proteins to efficiently slide along DNA.
Recommended Citation
Aleshintsev, Aleksey, Lindsey E. Way, Ngoc Khanh Lai, Bianca Guerra, Paloma Dorantes, Lois Akosua Serwaa, Sealtiel Anulao, Miranda Molina, Xindan Wang, and HyeongJun Kim. "Bacillus subtilis ParB C-terminal lysine residues are essential for dimerization and in vivo function, indicating their roles in DNA sliding." Nucleic Acids Research 54, no. 5 (2026): gkag202. https://doi.org/10.1093/nar/gkag202
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Publication Title
Nucleic Acids Research
DOI
10.1093/nar/gkag202
Included in
Astrophysics and Astronomy Commons, Biochemistry, Biophysics, and Structural Biology Commons, Biology Commons, Cell and Developmental Biology Commons, Physics Commons
Comments
© The Author(s) 2026. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.