Crotalus scutulatus scutulatus Crude Venom and Its Purified Cysteine-Rich Secretory Protein (Css-CRiSP)

Authors

Armando Reyes, Texas A & M University - Kingsville
Joseph D. Hatcher, Texas A & M University - Kingsville
Emelyn Salazar, Texas A & M University - Kingsville
Jacob Galan, The University of Texas Rio Grande ValleyFollow
Anton Iliuk
Elda E. Sanchez, Texas A & M University - Kingsville
Montamas Suntravat, Texas A & M University - Kingsville

Document Type

Article

Publication Date

7-2-2023

Abstract

Increased vascular permeability is a frequent outcome of viperid snakebite envenomation, leading to local and systemic complications. We reported that snake venom cysteine-rich secretory proteins (svCRiSPs) from North American pit vipers increase vascular permeability both in vitro and in vivo. They also induce acute activation of several adhesion and signaling molecules that may play a critical role in the pathophysiology of snakebites. Extracellular vesicles (EVs) have gained interest for their diverse functions in intercellular communication, regulating cellular processes, blood-endothelium interactions, vascular permeability, and immune modulation. They also hold potential as valuable biomarkers for diagnosing, predicting, and monitoring therapeutic responses in different diseases. This study aimed to identify proteins in peritoneal exudate and plasma EVs isolated from BALB/c mice following a 30 min post-injection of Crotalus scutulatus scutulatus venom and its purified CRiSP (Css-CRiSP). EVs were isolated from these biofluids using the EVtrap method. Proteomic analysis of exudate- and plasma-derived EVs was performed using LC-MS/MS. We observed significant upregulation or downregulation of proteins involved in cell adhesion, cytoskeleton rearrangement, signal transduction, immune responses, and vesicle-mediated transports. These findings suggest that svCRiSPs play a crucial role in the acute effects of venom and contribute to the local and systemic toxicity of snakebites.

Comments

© 2023 by the authors. Licensee MDPI, Basel, Switzerland.

Recommended Citation

Reyes, A., Hatcher, J. D., Salazar, E., Galan, J., Iliuk, A., Sanchez, E. E., & Suntravat, M. (2023). Proteomic Profiling of Extracellular Vesicles Isolated from Plasma and Peritoneal Exudate in Mice Induced by Crotalus scutulatus scutulatus Crude Venom and Its Purified Cysteine-Rich Secretory Protein (Css-CRiSP). Toxins, 15(7), 434. https://doi.org/10.3390/toxins15070434

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Publication Title

Toxins

DOI

10.3390/toxins15070434

Academic Level

faculty

Mentor/PI Department

Office of Human Genetics