Differential Methylation in APOE (Chr19; Exon Four; from 44,909,188 to 44,909,373/hg38) and Increased Apolipoprotein E Plasma Levels in Subjects with Mild Cognitive Impairment

Authors

Oscar Mancera-Páez
Kelly Estrada-Orozco
María Fernanda Mahecha
Francy Cruz
Kely Bonilla-Vargas
Nicolás Sandoval
Esneyder Guerrero
David Salcedo-Tacuma
Jesus Melgarejo, The University of Texas Rio Grande ValleyFollow
Edwin Vega

Document Type

Article

Publication Date

3-20-2019

Abstract

Background: Biomarkers are essential for identification of individuals at high risk of mild cognitive impairment (MCI) for potential prevention of dementia. We investigated DNA methylation in the APOE gene and apolipoprotein E (ApoE) plasma levels as MCI biomarkers in Colombian subjects with MCI and controls. Methods: In total, 100 participants were included (71% women; average age, 70 years; range, 43–91 years). MCI was diagnosed by neuropsychological testing, medical and social history, activities of daily living, cognitive symptoms and neuroimaging. Using multivariate logistic regression models adjusted by age and gender, we examined the risk association of MCI with plasma ApoE and APOE methylation. Results: MCI was diagnosed in 41 subjects (average age, 66.5 ± 9.6 years) and compared with 59 controls. Elevated plasma ApoE and APOE methylation of CpGs 165, 190, and 198 were risk factors for MCI (p < 0.05). Higher CpG-227 methylation correlated with lower risk for MCI (p = 0.002). Only CpG-227 was significantly correlated with plasma ApoE levels (correlation coefficient = −0.665; p = 0.008). Conclusion: Differential APOE methylation and increased plasma ApoE levels were correlated with MCI. These epigenetic patterns require confirmation in larger samples but could potentially be used as biomarkers to identify early stages of MCI.

Comments

© 2019 by the authors.

Recommended Citation

Mancera-Páez, O., Estrada-Orozco, K., Mahecha, M. F., Cruz, F., Bonilla-Vargas, K., Sandoval, N., ... & Arboleda, H. (2019). Differential methylation in APOE (Chr19; Exon four; from 44,909,188 to 44,909,373/hg38) and increased apolipoprotein E plasma levels in subjects with mild cognitive impairment. International journal of molecular sciences, 20(6), 1394. https://doi.org/10.3390/ijms20061394

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Publication Title

International journal of molecular sciences

DOI

10.3390/ijms20061394

Academic Level

faculty

Mentor/PI Department

Neuroscience