School of Medicine Publications and Presentations
Document Type
Article
Publication Date
12-18-2023
Abstract
A Bacille Calmette–Guérin (BCG) is still the only licensed vaccine for the prevention of tuberculosis, providing limited protection against Mycobacterium tuberculosis infection in adulthood. New advances in the delivery of DNA vaccines by electroporation have been made in the past decade. We evaluated the safety and immunogenicity of the DNA-hsp65 vaccine administered by intramuscular electroporation (EP) in cynomolgus macaques. Animals received three doses of DNA-hsp65 at 30-day intervals. We demonstrated that intramuscular electroporated DNA-hsp65 vaccine immunization of cynomolgus macaques was safe, and there were no vaccine-related effects on hematological, renal, or hepatic profiles, compared to the pre-vaccination parameters. No tuberculin skin test conversion nor lung X-ray alteration was identified. Further, low and transient peripheral cellular immune response and cytokine expression were observed, primarily after the third dose of the DNA-hsp65 vaccine. Electroporated DNA-hsp65 vaccination is safe but provides limited enhancement of peripheral cellular immune responses. Preclinical vaccine trials with DNA-hsp65 delivered via EP may include a combination of plasmid cytokine adjuvant and/or protein prime–boost regimen, to help the induction of a stronger cellular immune response.
Recommended Citation
de Lima, M. R., Leandro, A. C. C. S., de Souza, A. L., Barradas, M. M., Roma, E. H., Fernandes, A. T. G., Galdino-Silva, G., Carvalho, J. K. M. R., Marchevsky, R. S., Coelho, J. M. C. O., Gonçalves, E. D. C., VandeBerg, J. L., Silva, C. L., & Bonecini-Almeida, M. D. G. (2023). Safety and Immunogenicity of an In Vivo Muscle Electroporation Delivery System for DNA-hsp65 Tuberculosis Vaccine in Cynomolgus Monkeys. Vaccines, 11(12), 1863. https://doi.org/10.3390/vaccines11121863
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Publication Title
Vaccines
DOI
10.3390/vaccines11121863
Academic Level
faculty
Mentor/PI Department
Office of Human Genetics
Comments
© 2023 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).