School of Medicine Publications and Presentations
Document Type
Article
Publication Date
8-25-2018
Abstract
The therapeutic application of microRNA(s) in the field of cancer has generated significant attention in research. Previous studies have shown that miR-205 negatively regulates prostate cancer cell proliferation, metastasis, and drug resistance. However, the delivery of miR-205 is an unmet clinical need. Thus, the development of a viable nanoparticle platform to deliver miR-205 is highly sought. A novel magnetic nanoparticle (MNP)-based nanoplatform composed of an iron oxide core with poly(ethyleneimine)-poly(ethylene glycol) layer(s) was developed. An optimized nanoplatform composition was confirmed by examining the binding profiles of MNPs with miR-205 using agarose gel and fluorescence methods. The novel formulation was applied to prostate cancer cells for evaluating cellular uptake, miR-205 delivery, and anticancer, antimetastasis, and chemosensitization potentials against docetaxel treatment. The improved uptake and efficacy of formulations were studied with confocal imaging, flow cytometry, proliferation, clonogenicity, Western blot, q-RT-PCR, and chemosensitization assays. Our findings demonstrated that the miR-205 nanoplatform induces significant apoptosis and enhancing chemotherapeutic effects in prostate cancer cells. Overall, these study results provide a strong proof-of-concept for a novel nonviral-based nanoparticle protocol for effective microRNA delivery to prostate cancer cells.
Recommended Citation
Nagesh, P., Chowdhury, P., Hatami, E., Boya, V., Kashyap, V. K., Khan, S., Hafeez, B. B., Chauhan, S. C., Jaggi, M., & Yallapu, M. M. (2018). miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy. Cancers, 10(9), 289. https://doi.org/10.3390/cancers10090289
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Publication Title
Cancers
DOI
10.3390/cancers10090289
Academic Level
faculty
Mentor/PI Department
Immunology and Microbiology
Comments
© 2018 by the authors. Licensee MDPI, Basel, Switzerland