Tannic Acid Exhibits Antiangiogenesis Activity in Nonsmall-Cell Lung Cancer Cells

Authors

Elham Hatami, University of Tennessee Health Science Center
Prashanth K.B. Nagesh, The University of Texas Rio Grande Valley
Mohammed Sikander, The University of Texas Rio Grande Valley
Anupam Dhasmana, The University of Texas Rio Grande ValleyFollow
Subhash C. Chauhan, The University of Texas Rio Grande ValleyFollow
Meena Jaggi, The University of Texas Rio Grande Valley
Murali M. Yallapu, The University of Texas Rio Grande ValleyFollow

Document Type

Article

Publication Date

6-28-2022

Abstract

Nonsmall-cell lung cancer (NSCLC) is the most common type of lung cancer, with a dismal prognosis. NSCLC is a highly vascularized tumor, and chemotherapy is often hampered by the development of angiogenesis. Therefore, suppression of angiogenesis is considered a potential treatment approach. Tannic acid (TA), a natural polyphenol, has been demonstrated to have anticancer properties in a variety of cancers; however, its angiogenic properties have yet to be studied. Hence, in the current study, we investigated the antiproliferative and antiangiogenic effects of TA on NSCLC cells. The (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) assay revealed that TA induced a dose- and time-dependent decrease in the proliferation of A549 and H1299 cells. However, TA had no significant toxicity effects on human bronchial epithelial cells. Clonogenicity assay revealed that TA suppressed colony formation ability in NSCLC cells in a dose-dependent manner. The anti-invasiveness and antimigratory potential of TA were confirmed by Matrigel and Boyden chamber studies, respectively. Importantly, TA also decreased the ability of human umbilical vein endothelial cells (HUVEC) to form tube-like networks, demonstrating its antiangiogenic properties. Extracellular vascular endothelial growth factor (VEGF) release was reduced in TA-treated cells compared to that in control cells, as measured by the enzyme-linked immunosorbent assay (ELISA). Overall, these results demonstrate that TA can induce antiproliferative and antiangiogenic effects against NSCLC.

Comments

© 2022 The Authors. Published by American Chemical Society

Recommended Citation

Hatami, E., B Nagesh, P. K., Sikander, M., Dhasmana, A., Chauhan, S. C., Jaggi, M., & Yallapu, M. M. (2022). Tannic Acid Exhibits Antiangiogenesis Activity in Nonsmall-Cell Lung Cancer Cells. ACS omega, 7(27), 23939–23949. https://doi.org/10.1021/acsomega.2c02727

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.

Publication Title

ACS Omega

DOI

10.1021/acsomega.2c02727

Academic Level

faculty

Mentor/PI Department

Immunology and Microbiology