Presentation Type
Oral Presentation
Discipline Track
Biomedical Science
Abstract Type
Research/Clinical
Abstract
Background: The drug resistance in the microbes is a serious concern in medicine. Along with intrinsic factors, extrinsic factors like unprescribed usage of drugs are the contributing factors. The drug tolerance has led to the emergence of superbugs. Mycobacterial species utilize an array of multidrug efflux mechanisms linked to intrinsic and acquired antibiotic resistance. Understanding molecular mechanisms regulating efflux could reveal new therapeutic targets and strategies. Our study is aimed to target regulators of efflux Mycobacterial transporter.
Methods: Using the reference mycobacterial strain, antibiotic sensitivity was first profiled by minimal inhibitory concentration assays across a panel of antimicrobials, followed by mutagenesis analyses of transporter-deficient mutants. To uncover regulators, real-time transcriptional induction in the presence of selected antimicrobials is being done.
Results: Measurements of antibiotic susceptibility in mycobacterial strains are anticipated to demonstrate reduced drug tolerance. There are dose-dependent associations of transcriptional activation of efflux genes paving way for elucidating mechanisms governing anti-microbial efflux. This work would provide candidate targets to block resistance emergence strains.
Conclusions: Identifying and targeting regulators of mycobacterial efflux systems may enable novel therapeutic approaches countering antimicrobial resistance, with wider applicability across nosocomial and community-acquired infections. It leads to drug development strategies against mycobacterial infections.
Academic/Professional Position
Community Partner
Recommended Citation
Radhakrishnan, Arathi; Kapardar, Raj Kishor; and Srivastav, Rajpal, "Targeting mycobacterial efflux system for combating anti-microbial resistance" (2024). Research Symposium. 27.
https://scholarworks.utrgv.edu/somrs/2024/talks/27
Targeting mycobacterial efflux system for combating anti-microbial resistance
Background: The drug resistance in the microbes is a serious concern in medicine. Along with intrinsic factors, extrinsic factors like unprescribed usage of drugs are the contributing factors. The drug tolerance has led to the emergence of superbugs. Mycobacterial species utilize an array of multidrug efflux mechanisms linked to intrinsic and acquired antibiotic resistance. Understanding molecular mechanisms regulating efflux could reveal new therapeutic targets and strategies. Our study is aimed to target regulators of efflux Mycobacterial transporter.
Methods: Using the reference mycobacterial strain, antibiotic sensitivity was first profiled by minimal inhibitory concentration assays across a panel of antimicrobials, followed by mutagenesis analyses of transporter-deficient mutants. To uncover regulators, real-time transcriptional induction in the presence of selected antimicrobials is being done.
Results: Measurements of antibiotic susceptibility in mycobacterial strains are anticipated to demonstrate reduced drug tolerance. There are dose-dependent associations of transcriptional activation of efflux genes paving way for elucidating mechanisms governing anti-microbial efflux. This work would provide candidate targets to block resistance emergence strains.
Conclusions: Identifying and targeting regulators of mycobacterial efflux systems may enable novel therapeutic approaches countering antimicrobial resistance, with wider applicability across nosocomial and community-acquired infections. It leads to drug development strategies against mycobacterial infections.