Posters
Academic/Professional Position (Other)
Masters Student
Presentation Type
Poster
Discipline Track
Biomedical ENGR/Technology/Computation
Abstract Type
Research/Clinical
Abstract
Background: Homeoproteins are transcriptional factors (TFs) that shape animal body axes during development. These TFs are highly conserved and represent one of the most fascinating groups of regulatory molecules. Reports shown the multiplicity of interactions in hox proteins, as complexes trimeric involved to transcriptional activity. The study of trimeric complexes in Hox interactome will allow the better understanding of Hox genetic regulation during embryonic development.
Methodology: Using a new combination BiFCFRET approach performed in HEK293, the quantification was performed by FRETTY of ImageJ. Fly crosses were incubated at 25ºC on standard yeast-agar-cornmeal medium. Embryo cuticle preparations were carried out according to (Gibson & Gehring 1998) and mounted on slides with Visicol. For adult imaging, the heads and antennae were dissected and directly transferred to microscopic slides without coverslips. The images were merged using the software HeliconFocus.
Results: We found the presence of trimeric interactions of Antp-TBP-TFIIEβ and Antp-TBP-Exd in cell culture. Further were confirmed these trimeric interactions using Antp mutants. Interestingly, the trimeric Antp-TBP-TFIIEB shown diminish of homeotic effect caused by Antp in larvae. To analyze the effect in antenna-leg transformation we drive the trimeric complex and found inhibition of antenna-leg in head of D. melanogaster. Antp-TBP-TFIIEB shown homeotic reduction of 77% in larvae and found inhibition of 100% of antenna-leg transformation.
Conclusions: Our results show that these trimeric interactions are involved in the genetic control of Drosophila melanogaster.
Recommended Citation
Mejía, Gustavo Jiménez; Mendez, Ruben de Jesus Montalvo; Torres, Claudia Dalila Altamirano; and Pérez, Diana Reséndez, "Trimeric complex interactions of Antp-TBP with TFIIEB and Exd are involved in the genetic control of Drosophila melanogaster" (2023). Research Symposium. 103.
https://scholarworks.utrgv.edu/somrs/theme1/posters/103
Trimeric complex interactions of Antp-TBP with TFIIEB and Exd are involved in the genetic control of Drosophila melanogaster
Background: Homeoproteins are transcriptional factors (TFs) that shape animal body axes during development. These TFs are highly conserved and represent one of the most fascinating groups of regulatory molecules. Reports shown the multiplicity of interactions in hox proteins, as complexes trimeric involved to transcriptional activity. The study of trimeric complexes in Hox interactome will allow the better understanding of Hox genetic regulation during embryonic development.
Methodology: Using a new combination BiFCFRET approach performed in HEK293, the quantification was performed by FRETTY of ImageJ. Fly crosses were incubated at 25ºC on standard yeast-agar-cornmeal medium. Embryo cuticle preparations were carried out according to (Gibson & Gehring 1998) and mounted on slides with Visicol. For adult imaging, the heads and antennae were dissected and directly transferred to microscopic slides without coverslips. The images were merged using the software HeliconFocus.
Results: We found the presence of trimeric interactions of Antp-TBP-TFIIEβ and Antp-TBP-Exd in cell culture. Further were confirmed these trimeric interactions using Antp mutants. Interestingly, the trimeric Antp-TBP-TFIIEB shown diminish of homeotic effect caused by Antp in larvae. To analyze the effect in antenna-leg transformation we drive the trimeric complex and found inhibition of antenna-leg in head of D. melanogaster. Antp-TBP-TFIIEB shown homeotic reduction of 77% in larvae and found inhibition of 100% of antenna-leg transformation.
Conclusions: Our results show that these trimeric interactions are involved in the genetic control of Drosophila melanogaster.