Posters

Presenting Author

Daniel L. Shen

Presentation Type

Poster

Discipline Track

Clinical Science

Abstract Type

Research/Clinical

Abstract

Background: African-American (AA) men have higher incidence and mortality from prostate cancer compared to Caucasian-American (CA) men. Increasing evidence suggests that genetic and molecular alterations play important roles. We identified a 5 gene panel viz. p-Akt (Ser473), chemokine (C-X-C motif) receptor 4 (CXCR4), fatty acid synthase (FASN), interleukin-6 (IL-6) and matrix metallopeptidase 9 (MMP-9) highly expressed in prostate cancer and analyzed their expression in AA and CA cohorts.

Methods: IHC of p-Akt, CXCR4, FASN, IL-6 and MMP-9 were evaluated in RRP specimens (n=20) from each ethnic group exhibiting Gleason scores ranging from 6 through 9.

Results: Low to medium staining for p-Akt and weak focal staining for MMP-9 was observed in the cytoplasm of tumor cells (10-20%) in80% of tumor cells in both groups. Expression of IL-6 varied from weak to moderate intensity between (20-100% tumor cells) in 85% cases in CA- and 75% in AA- specimens. A marked difference in CXCR4 expression was noted between AA- and CA- cancer specimens. Weak CXCR4 staining was noted85% of AA- prostate cancer exhibited weak to strong CXCR4 expression in between 10-100% of tumor cells localized in membrane, cytoplasm and nucleus in high-grade tumors.

Conclusions: CXCR4 expression appears to be distinctly different in prostate cancers from AA and CA men. Further studies are needed to assess whether this distinction correlates with prognosis between racial groups.

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Differential gene expression between African American and Caucasian American prostate cancer

Background: African-American (AA) men have higher incidence and mortality from prostate cancer compared to Caucasian-American (CA) men. Increasing evidence suggests that genetic and molecular alterations play important roles. We identified a 5 gene panel viz. p-Akt (Ser473), chemokine (C-X-C motif) receptor 4 (CXCR4), fatty acid synthase (FASN), interleukin-6 (IL-6) and matrix metallopeptidase 9 (MMP-9) highly expressed in prostate cancer and analyzed their expression in AA and CA cohorts.

Methods: IHC of p-Akt, CXCR4, FASN, IL-6 and MMP-9 were evaluated in RRP specimens (n=20) from each ethnic group exhibiting Gleason scores ranging from 6 through 9.

Results: Low to medium staining for p-Akt and weak focal staining for MMP-9 was observed in the cytoplasm of tumor cells (10-20%) in80% of tumor cells in both groups. Expression of IL-6 varied from weak to moderate intensity between (20-100% tumor cells) in 85% cases in CA- and 75% in AA- specimens. A marked difference in CXCR4 expression was noted between AA- and CA- cancer specimens. Weak CXCR4 staining was noted85% of AA- prostate cancer exhibited weak to strong CXCR4 expression in between 10-100% of tumor cells localized in membrane, cytoplasm and nucleus in high-grade tumors.

Conclusions: CXCR4 expression appears to be distinctly different in prostate cancers from AA and CA men. Further studies are needed to assess whether this distinction correlates with prognosis between racial groups.

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