Posters
Presentation Type
Poster
Discipline Track
Translational Science
Abstract Type
Research/Clinical
Abstract
Background: Azadirachta indica (neem), a medicinal plant under Meliaceae family, is found in the Indian subcontinent. One of the limonoids, epoxyazadiradione (EPA), is a phytochemical isolated from the seeds of this tree. This is widely used in traditional medicine to treat a variety of human ailments. Although EPA has shown promise against some cancer types, its efficacy against pancreatic cancer and the underlying mechanism remains elusive.
Aim: We examined the anti‑cancer activity of EPA against pancreatic cancer cells. We also examined the underlying mechanism.
Methods: Pancreatic cancer cell lines (PANC-1 and MiaPaCa-2) were used during the study. We performed MTT assay, clonogenic colony formation assay for cytotoxicity. The western blotting was performed to examine the expression pattern of various apoptotic proteins. Real-time PCR was performed to detect quantitative lncRNAs expression.
Results and Discussion: After treatment with EPA, the viability and proliferation of pancreatic cancer cells was decreased in a dose- and time-dependent manner. EPA suppressed the expression of apoptotic proteins involved in survival, proliferation, migration and invasion. EPA also suppressed the expression of MMP-9 in a concentration-dependent manner in pancreatic cancer cells. In addition, the limonoid also modulated the expression of lncRNAs (MEG-3, GAS-5, H19 and MHRT).
Conclusion: EPA exhibited strong anti-cancer activities against pancreatic cancer by modulating multiple cancer-related signalling molecules.
Recommended Citation
Rai, Vipin; Awasthee, Nikee; Verma, Sumit Singh; and Gupta, Subash Chandra, "Epoxyazadiradione exhibit anti‑cancer activities by modulating lncRNAs expression in pancreatic cancer" (2023). Research Symposium. 48.
https://scholarworks.utrgv.edu/somrs/theme1/posters/48
Epoxyazadiradione exhibit anti‑cancer activities by modulating lncRNAs expression in pancreatic cancer
Background: Azadirachta indica (neem), a medicinal plant under Meliaceae family, is found in the Indian subcontinent. One of the limonoids, epoxyazadiradione (EPA), is a phytochemical isolated from the seeds of this tree. This is widely used in traditional medicine to treat a variety of human ailments. Although EPA has shown promise against some cancer types, its efficacy against pancreatic cancer and the underlying mechanism remains elusive.
Aim: We examined the anti‑cancer activity of EPA against pancreatic cancer cells. We also examined the underlying mechanism.
Methods: Pancreatic cancer cell lines (PANC-1 and MiaPaCa-2) were used during the study. We performed MTT assay, clonogenic colony formation assay for cytotoxicity. The western blotting was performed to examine the expression pattern of various apoptotic proteins. Real-time PCR was performed to detect quantitative lncRNAs expression.
Results and Discussion: After treatment with EPA, the viability and proliferation of pancreatic cancer cells was decreased in a dose- and time-dependent manner. EPA suppressed the expression of apoptotic proteins involved in survival, proliferation, migration and invasion. EPA also suppressed the expression of MMP-9 in a concentration-dependent manner in pancreatic cancer cells. In addition, the limonoid also modulated the expression of lncRNAs (MEG-3, GAS-5, H19 and MHRT).
Conclusion: EPA exhibited strong anti-cancer activities against pancreatic cancer by modulating multiple cancer-related signalling molecules.
