Talks
Microbiome dysbiosis in cervical cancer health disparities
Presentation Type
Oral Presentation
Discipline Track
Clinical Science
Abstract Type
Research/Clinical
Abstract
Background: Cervical cancer(CC)is a high-risk human papilloma virus (hrHPV) associated malignancy and one of the leading causes of cancer-related death in women in the USA and worldwide. Surveillance through hrHPV and pap smear-based testing has remarkably reduced CC incidence. However, considerable racial disparities in the incidence and clinical outcome of CC exists. Recent studies suggest that imbalance in cervical microbiome may play a crucial role in CC risk and outcome.
Methods: Cervical intraepithelial neoplasia (CIN)lesions were collected from African-American (AA), Caucasian-American (CA)and Hispanic/Latina (HIS)women (n=12 from each group, total =36). Bacterial genomic DNA was extracted from the above bio-specimens and the 16S rDNA V4 region was amplified Illumina 16Sv4 v1.2)by PCR and sequenced on the MiSeq platform (Illumina MiSeq v2 2x250 v1.8).
Results: A total of 232,095 reads and 259 unique operational taxonomic units (OTUs) were observed. OTUs were across 13 different phyla, 74 families, and 142 genera. The top phyla identified included Actinobacteria, Proteobacteria, Firmicutes, and Bacteroidetes. Three major genera identified were Lactobacillus, Leucobacter, and Corynebacterium. Among the genera that showed different read abundances across groups were Gardnerella, Micrococcus, and Prevotella. Lower abundance of Lactobacillus, a beneficial microbe of the female reproductive system was evident in both AA and HIS compared to CA women. On the other hand, a higher abundance of pathogenic Leucobacter and Prevotella was evident in the AA/HIS compared to CA women.
Conclusions: Presence of distinct microbial niche in precancerous cervical lesions from different racial groups may be associated with varying risk of developing CC and subsequent progression that should be explored in future investigations.
Academic/Professional Position
Faculty
Academic/Professional Position (Other)
PhD
Recommended Citation
Vikramdeo, Kunwar S.; Singh, Seema; Singh, Ajay P.; and Dasgupta, Santanu, "Microbiome dysbiosis in cervical cancer health disparities" (2023). Research Symposium. 57.
https://scholarworks.utrgv.edu/somrs/theme1/track1/57
Microbiome dysbiosis in cervical cancer health disparities
Background: Cervical cancer(CC)is a high-risk human papilloma virus (hrHPV) associated malignancy and one of the leading causes of cancer-related death in women in the USA and worldwide. Surveillance through hrHPV and pap smear-based testing has remarkably reduced CC incidence. However, considerable racial disparities in the incidence and clinical outcome of CC exists. Recent studies suggest that imbalance in cervical microbiome may play a crucial role in CC risk and outcome.
Methods: Cervical intraepithelial neoplasia (CIN)lesions were collected from African-American (AA), Caucasian-American (CA)and Hispanic/Latina (HIS)women (n=12 from each group, total =36). Bacterial genomic DNA was extracted from the above bio-specimens and the 16S rDNA V4 region was amplified Illumina 16Sv4 v1.2)by PCR and sequenced on the MiSeq platform (Illumina MiSeq v2 2x250 v1.8).
Results: A total of 232,095 reads and 259 unique operational taxonomic units (OTUs) were observed. OTUs were across 13 different phyla, 74 families, and 142 genera. The top phyla identified included Actinobacteria, Proteobacteria, Firmicutes, and Bacteroidetes. Three major genera identified were Lactobacillus, Leucobacter, and Corynebacterium. Among the genera that showed different read abundances across groups were Gardnerella, Micrococcus, and Prevotella. Lower abundance of Lactobacillus, a beneficial microbe of the female reproductive system was evident in both AA and HIS compared to CA women. On the other hand, a higher abundance of pathogenic Leucobacter and Prevotella was evident in the AA/HIS compared to CA women.
Conclusions: Presence of distinct microbial niche in precancerous cervical lesions from different racial groups may be associated with varying risk of developing CC and subsequent progression that should be explored in future investigations.